不同硒浓度日粮对小鼠肝脏和睾丸组织中部分硒蛋白mRNA水平的影响

Abstract

Abstract: A total of 80 7-week-old male BALB/c mice were randomly divided into four groups (Ⅰ,Ⅱ,Ⅲ and Ⅳ groups),and 20 mice in each group. Each group was fed with the diet containing 0.045,0.1,0.4,and 0.8 mg/kg Se,respectively. The results showed that the content Se of Ⅰ group in the liver reduced significantly (P<0.05). Glutathione peroxidase 1 (GPx1)mRNA levels of Ⅰ group was significant lower than Ⅱ and Ⅲ groups (P<0.05),while Ⅱ and Ⅲ groups were significant differences in the liver (P<0.05),and no significant differences in testis (P>0.05). Glutathione peroxidase 4 (GPx4) mRNA level of Ⅰand Ⅲ groups in liver had no significant difference (P>0.05),but were significant lower than group Ⅱ (P<0.05),while each group had no difference in the testis (P>0.05). Relative mRNA expression of thioredoxin reductase 2 (TrxR2) in liver and testis improved significantly (P<0.05) with the increasing of dietary Se supplemention. In conclusion,in liver and testis,deficient Se dietary could significantly reduce selenium deposition and relative mRNA expression of GPx1,GPx4 and TrxR2,however,excessive Se dietary could significantly reduce GPx1 mRNA levels (P<0.05) and significantly increase TrxR2 mRNA levels (P<0.05),it showed that there was difference for different organs.

Key words: selenium(Se); glutathione peroxidase 1; glutathione peroxidase 4; thioredoxin reductase 2; mRNA; liver; testis

CLC Number:

S816.71

ZHENG Liang-yan, ZHANG Qin, LIU Bi-tao, GUO Kai, YU Wen-lan, LIU Zheng-wei, CHEN Ye, GUO Cui-li, TANG Zhao-xin. Effect of Different Dietary Selenium Concentration on mRNA Levels of Some Selenoprotein in Mice Liver and Testis[J]. , 2013, 40(3): 38-42.

References

1 化罗明,徐光禄,谭武红,等.补硒对低硒居民血小板活化状态的影响[J].营养学报,1997,19(2):167～171.
2 王广珠,牛作霞.微量元素硒的毒性研究进展[J].西北药学杂志,2010(3):237～238.
3 王晓雅.硒蛋白的抗氧化作用[J].现代农业科技,2006(8):183～184.
4 王海娜,许厚强,张勇,等.有机硒对雄性昆明小鼠繁殖性能影响的研究[J].中国畜牧兽医,2011,38(4):140～143.
5 张巧娥,顾亚玲,杨库,等.不同硒水平对生长育肥猪血液生化指标的影响[J].畜牧与兽医,2003(10):16～19.
6 高良才,陈婉蓉,傅中滇,等.硒对衰老小鼠脑神经细胞及线粒体的保护作用[J].中国公共卫生,2002(7):5～6.
7 Blodgett D J.Vitamin E-selenium and immune response to selected antigens in swine[J].Vet Med,1984,32:452～457.
8 Burk F R,Hill K E.Regulation of selenoproteins[J].Annual Review of Nutrition,1993,13:65～81.
9 Lin F P.Advance in selenium nutrition research[J].Fujian Anim Sci Vet Med,2002,2:45～51.
10 Reeves P G,Nielsen F H,Fahey G C Jr.AIN-93 purified diets for laboratory rodents:Final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].J Nutr,1993,123(11):1939～1951.
11 Rotruck J T,Pope A L,Garther H E,et al.Selenium: Biochem role as a component of glutathine peroxidase[J].Science,1973,179:588～590.
12 Rundlo F A K,Arner E S.Regulation of the mammalian seleoprotein thioredoxin reductase 1 in relation to cellular phenotype,growth,and signaling events[J].Antioxid Redox Signal,2004,6:41～52.
13 Schwarz K,Foltz C M.Selenium as an integral part of factor 3 against dietary necrotic liver degeneration[J].Nutrition,1999,15(3):255.
14 Song J J,Lee Y J.Differential role of glutaredoxin and thioredoxin in metabolic oxidative stress induced activation of apoptosis signal regulating kinase 1[J].Biochem J,2003,373(3):845～853.
15 Sun Q,Kojima H,Setal K. Effect of selenium on human phospholipids hydroperoxide glutathione peroxidase expression and host cell susceptibility to lipid hydroperoxide-mediated injury[J].Biochem Mol Biol Int,1997,42(5):957～961.

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Effect of Different Dietary Selenium Concentration on mRNA Levels of Some Selenoprotein in Mice Liver and Testis

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