ABCG2基因不同基因型对蛋鸭蛋壳颜色、蛋品质和产蛋性能的影响

doi:10.16431/j.cnki.1671-7236.2025.04.020

Abstract

Abstract: 【Objective】 The genotype of ATP-binding cassette G subfamily member 2 (ABCG2) gene was identified and green-shell homozygous M11 Peking ducks (60-week-old) were screened.The effects of different genotypes of ABCG2 gene on eggshell color,biliverdin synthesis ability,egg quality and laying performance were explored.【Method】 60-week-old M11 Peking ducks were used as the experimental population.The genotype of ABCG2 gene was identified by bidirectional allele-specific PCR (Bi-PASA) technology,and were verified by Sanger sequencing.Starting from the first day of 60 weeks of age,duck eggs were collected continuously for 5 days to measure the egg quality,eggshell color grades,red value (R value),green value (G value),blue value (B value) and biliverdin content.Ten ducks were randomly selected from different genotype populations,and collected their livers and eggshell glands.The biliverdin content in the liver and eggshell glands,as well as the expression of genes related to biliverdin synthesis in the eggshell gland were measured.【Result】 Bi-PASA genotyping results were consistent with Sanger sequencing.The frequencies of AA,AG and GG genotypes in the population were 68.97%,26.49% and 4.55%,respectively,and there was a white shell rate of 4.55%.The egg shape index of duck eggs produced by AG genotype female ducks were significantly higher than those of AA genotype,their Haugh unit was significantly smaller than that of AA genotype,and the egg weight was significantly higher than that of AA and GG genotypes (P＜0.05).The eggshell color grades and biliverdin content of duck eggs produced by AA and AG genotype female ducks were significantly higher than that of GG genotype (P＜0.05),and the R and B values of eggshells were significantly lower than those of GG genotype (P＜0.05),but there was no significant difference between AG and AA genotypes (P＞0.05).The biliverdin contents of the liver and eggshell glands of AA and AG genotype female ducks were significantly or extremely significantly higher than those of GG genotype (P＜0.05 or P＜0.01)，the expression levels of 5'-aminolevulinic acid synthase 1 (ALAS1),fecal porphyrinogen oxidase (CPOX) and ABCG2 genes in eggshell gland were significantly or extremely significantly higher than those in GG genotype (P＜0.05 or P＜0.01) and the gene expression level of biliverdin reductase A (BLVRA) was extremely significantly lower than that of GG genotype (P＜0.01),but there was no significant difference between AG and AA genotypes (P＞0.05).【Conclusion】 Bi-PASA technology could accurately determine the ABCG2 genotype.The genotype of ABCG2 gene could significantly affect the color grade,R value,G value,B value and biliverdin content of duck egg shells.Duck eggs produced by AA and AG genotype female ducks were all green.The eggs produced by the GG genotype female ducks were all white.The AA and AG genotype female ducks had stronger biliverdin synthesis and transport abilities,but the eggs produced by the AA and AG genotype female ducks all had very light or extremely green eggs.The results provided theoretical basis and practical reference for breeding white-feathered and green-shelled duck strains.

Key words: duck; eggshell color; ABCG2 gene; genotype; biliverdin; egg quality; laying performance

CLC Number:

S834

WANG Longxin, YU Simeng, LU Meixi, BAI Xiangxing, HOU Shuisheng. Effects of Different Genotypes of ABCG2 Gene on Eggshell Color, Egg Quality and Laying Performance of Laying Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1671-1680.

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Effects of Different Genotypes of ABCG2 Gene on Eggshell Color, Egg Quality and Laying Performance of Laying Ducks

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