藏鸡胚胎不同发育阶段腿肌组织中miRNA的分析及鉴定

doi:10.16431/j.cnki.1671-7236.2025.04.021

Abstract

Abstract: 【Objective】 This study was aimed to explore microRNA (miRNA) related to skeletal muscle growth and development during the embryonic period of Tibetan chickens,analyze their differential expression and construct the miRNA-mRNA regulatory network,so as to provide a theoretical basis for further understanding the regulatory mechanism of miRNA in the growth and development of chicken muscles.【Method】 RNA-Seq was performed on the leg muscle tissues of Tibetan chickens at the 10th (E10),14th (E14) and 18th (E18) days of embryonic stage.The differentially expressed miRNA among three periods was screened through bioinformatics analysis,and the differentially expressed miRNA was enriched by GO function and KEGG pathway,and the mRNA and miRNA related to muscle development were screened to construct the mRNA-miRNA regulatory network.Six differentially expressed miRNAs were randomly selected for Real-time quantitative PCR to verify RNA-Seq results.【Result】 The results showed that 52 miRNAs were differentially expressed between E10 and E14,137 miRNAs were differentially expressed between E10 and E18,33 miRNAs were differentially expressed between E14 and E18,and 8 miRNAs were differentially expressed in leg muscles of Tibetan chickens among three periods.The results of GO function enrichment analysis showed that the items related to skeletal muscle growth and development were actin binding,myofibrillar assembly,actomyosin structure and actin filament-based processes,muscle fiber membrane,etc.KEGG pathway enrichment analysis showed that a large number of genes were significantly enriched in the signaling pathways related to the biological functions of cell adhesion,survival,migration,proliferation,metabolism and differentiation,and immune regulation.The results of miRNA-3P-MYO1D/PDLIM3,miRNA-1662-DMD/SYNM,and miRNA-184-3p-FHOD1 might play an important role in the muscle growth and development of chicken embryos.miRNAs such as miRNA-133c-3p,miRNA-184-3p,miRNA-1662,miRNA-210a-3p and miRNA-1a-3p might be involved in the process of muscle growth and development.Real-time quantitative PCR results showed that the expression of six miRNAs were consistent with RNA-Seq results.【Conclusion】 A total of 222 differentially expressed miRNAs were detected,among which 8 miRNAs were differentially expressed at all three periods,providing theoretical basis for subsequent studies on the regulation of miRNA on muscle growth and development in chicken embryos.

Key words: Tibetan chickens; embryonic period; muscle development; miRNA; RNA-Seq

CLC Number:

S831.2

LI Zhiyi, LI Jie, CHEN Chuwen, NONG Yi, WANG Jiayan, WANG Zi, WU Jinbo, LI Zhixiong. Analysis and Identification of miRNA in Leg Muscle Tissue of Tibetan Chicken Embryos at Different Developmental Stages[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1681-1693.

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Analysis and Identification of miRNA in Leg Muscle Tissue of Tibetan Chicken Embryos at Different Developmental Stages

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