罗伊氏乳杆菌替代抗生素对仔猪肠道miRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2024.01.008

Abstract

Abstract: 【Objective】 This study was conducted to investigate the effects of Lactobacillus reuteri LR1 replacing antibiotics on the expression of miRNA in different intestinal segments of piglets, so as to screen potential miRNA for studying the mechanism of Lactobacillus reuteri LR1 on regulating intestinal function of piglets.【Method】 A total of 144 cross-bred (Duroc×Landrace×Yorkshire) weaned piglets with similar body weight at 21 days old were selected and randomly divided into 3 groups, with 8 replicates in each group and 6 piglets in each replicate.The piglets in control group were fed with the basic diet, the piglets in antibiotic and probiotic groups were fed with the basic diet supplemented with compound antibiotics (100 mg/kg quinoline ethanol+75 mg/kg aureomycin) and 5×10¹⁰ CFU Lactobacillus reuteri LR1, respectively.The experiment lasted for 42 days.At the end of the experiment, 1 piglet from each replicate was randomly selected and slaughtered, the jejunum tissue was collected for miRNA high-throughput sequencing and analysis.The expression of the selected miRNA in duodenum, jejunum, ileum and colon were detected by Real-time quantitative PCR.【Result】 Lactobacillus reuteri LR1 supplementation caused significant changes in miRNA expression, altering the sort of highly expressed miRNA in jejunum.The potential target genes of differentially expressed miRNA were mainly enriched in metabolic related pathways, PI3K-Akt, MAPK, Chemokine and Ras signaling pathways.Real-time quantitative PCR results showed that the expression of differential miRNA in jejunum among 3 groups had the same result with RNA-Seq.Moreover, the expression of up-regulated miRNA showed a relatively consistent trend between control and antibiotic groups, down-regulated miRNA showed a relatively consistent trend between control and probiotic groups, while the highly expressed miRNA in intestine showed a relatively consistent expression trend among 3 groups.【Conclusion】 Adding Lactobacillus reuteri LR1 in diet replacing antibiotics could cause changes in the expression of intestinal miRNA.The differentially expressed miRNA screened could provide a foundation for research on the regulating intestinal development and nutrient absorption metabolism by Lactobacillus reuteri LR1 in piglets.

Key words: Lactobacillus reuteri; piglets; intestinal miRNA

CLC Number:

S816.7

LI Hongyi, YANG Xuefen, LIN Zesen, WU Xiaofei, LYU Qixin, YU Jiali, ZHANG Mao. Effect of Lactobacillus reuteri Replacing Antibiotics on the Expression of Intestinal miRNA in Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 74-85.

References

[1] 杨广达, 王丽, 易宏波, 等.罗伊氏乳杆菌LR1对断奶仔猪血清生化指标和肠道营养物质转运载体mRNA表达的影响[J].动物营养学报, 2018, 30(11):4589-4600. YANG G D, WANG L, YI H B, et al.Effects of Lactobacillus reuteri LR1 on serum biochemical indexes and mRNA expressions of intestinal nutrient transporters of weaned piglets[J].Chinese Journal of Animal Nutrition, 2018, 30(11):4589-4600.(in Chinese)
[2] YI H, YANG G, XIONG Y, et al.Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs[J].Food & Function, 2019, 10(11):7387-7396.
[3] TIAN Z, CUI Y, LU H, et al.Effect of long-term dietary probiotic Lactobacillus reuteri 1 or antibiotics on meat quality, muscular amino acids and fatty acids in pigs[J].Meat Science, 2021, 171:108234.
[4] GAO J C, CAO S T, XIAO H, et al.Lactobacillus reuteri 1 enhances intestinal epithelial barrier function and alleviates the inflammatory response induced by enterotoxigenic Escherichia coli K88 via suppressing the MLCK signaling pathway in IPEC-J2 cells[J].Frontiers in Immunology, 2022, 13:897395.
[5] DELL'ANNO M, CALLEGARI M L, REGGI S, et al.Lactobacillus plantarum and Lactobacillus reuteri as functional feed additives to prevent diarrhoea in weaned piglets[J].Animals (Basel), 2021, 11(6):1766.
[6] YANG B, LIU C, HUANG Y, et al.The responses of Lactobacillus reuteri LR1 or antibiotic on intestinal barrier function and microbiota in the cecum of pigs[J].Frontiers in Microbiology, 2022, 13:877297.
[7] 冯军森, 熊云霞, 吴绮雯, 等.饲粮添加罗伊氏乳杆菌LR1对猪常见病毒疫苗免疫效果的影响[J].中国畜牧兽医, 2020, 47(3):884-891. FENG J S, XIONG Y X, WU Q X, et al.The effect of Lactobacillus reuteri LR1 on the immune response of swine common virus vaccine[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(3):884-891.(in Chinese)
[8] BHANJA P, NORRIS A, GUPTA-SARAF P, et al.BCN057 induces intestinal stem cell repair and mitigates radiation-induced intestinal injury[J].Stem Cell Research & Therapy, 2018, 9(1):26.
[9] ANDRIATSILAVO M, STEFANUTTI M, SIUDEJA K, et al.Spen limits intestinal stem cell self-renewal[J].PLoS Genetics, 2018, 14(11):e1007773.
[10] RAMIRO-CORTIJO D, SINGH P, LIU Y, et al.Breast milk lipids and fatty acids in regulating neonatal intestinal development and protecting against intestinal injury[J].Nutrients, 2020, 12(2):534.
[11] NOSALSKI R, SIEDLINSKI M, DENBY L, et al.T-cell-derived miRNA-214 mediates perivascular fibrosis in hypertension[J].Circulation Research, 2020, 126(8):988-1003.
[12] 孙倩, 王琪, 黄金秀, 等.罗伊氏乳杆菌对仔猪空肠miRNAs表达谱的影响[J].畜牧兽医学报, 2020, 51(9):2156-2164. SUN Q, WANG Q, HUANG J X, et al.Effect of Lactobacillus reuteri on the expression profile of miRNAs in the jejunum of piglets[J].Acta Veterinaria et Zootechnica Sinica, 2020, 51(9):2156-2164.(in Chinese)
[13] WANG Z L, WANG L, CHEN Z, et al.In vitro evaluation of swine-derived Lactobacillus reuteri:Probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic Escherichia coli K88[J].Journal of Microbiology and Biotechnology, 2016, 26(6):1018-1025.
[14] ROBINSON M D, MCCARTHY D J, SMYTH G K.edgeR:A Bioconductor package for differential expression analysis of digital gene expression data[J].Bioinformatics, 2010, 26(1):139-140.
[15] ZOU L, XIONG X, WANG K, et al.microRNAs in the intestine:Role in renewal, homeostasis, and inflammation[J].Current Molecular Medicine, 2018, 18(3):190-198.
[16] DHUPPAR S, MURUGAIYAN G.miRNA effects on gut homeostasis:Therapeutic implications for inflammatory bowel disease[J].Trends in Immunology, 2022, 43(11):917-931.
[17] WANG Z Q, WANG B, WANG K.Targeting TRIM9 by miR-218-5p restricts cell proliferation and epithelial-mesenchymal transition in non-small cell lung cancer[J].Annals of Clinical and Laboratory Science, 2023, 53(1):106-115.
[18] WISCHMANN F J, TROSCHEL F M, FRANKENBERG M, et al.Tumor suppressor miR-218 directly targets epidermal growth factor receptor (EGFR) expression in triple-negative breast cancer, sensitizing cells to irradiation[J].Journal of Cancer Research and Clinical Oncology, 2023, 149(11):8455-8465.
[19] GRIELA E, MOUNTZOURIS K C.Nutrigenomic profiling of reduced specification diets and phytogenic inclusion effects on critical Toll-like receptor signaling, mitogen-activated protein kinase-apoptosis, and PI3K-Akt-mTOR gene components along the broiler gut[J].Journal of Poultry Science, 2023, 102(6):102675.
[20] SUN L, WU S, DAI C H, et al.Insight into the molecular mechanism of miR-192 regulating Escherichia coli resistance in piglets[J].Bioscience Reports, 2018, 38(1):BSR20171160.
[21] TAO X, XU Z.MicroRNA transcriptome in swine small intestine during weaning stress[J].PLoS One, 2013, 8(11):e79343.
[22] PAREEK S, SANCHENKOVA X, SAKAGUCHI T, et al.Epithelial miR-215 negatively modulates Th17-dominant inflammation by inhibiting CXCL12 production in the small intestine[J].Genes to Cells, 2022, 27(4):243-253.
[23] LI H Y.Effect of microRNA, miR-215, on gene expression and metabolism of porcine intestinal epithelial cells[J].South African Journal of Animal Science, 2022, 6(52):886-899.
[24] CAO J, HUO P, CUI K, et al.Follicular fluid-derived exosomal miR-143-3p/miR-155-5p regulate follicular dysplasia by modulating glycolysis in granulosa cells in polycystic ovary syndrome[J].Cell Communication and Signaling, 2022, 20(1):61.

Effect of Lactobacillus reuteri Replacing Antibiotics on the Expression of Intestinal miRNA in Piglets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Chunyan, TANG Qingsong, YANG Bijing, WU Qiwen, XIONG Yunxia, HUANG Yanna, YANG Xuefen, WANG Li, JIANG Zongyong, YI Hongbo. Effects of Enterotoxigenic Escherichia coli on Intestinal Morphology, Tight Junction Protein and Extracellular Matrix of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2697-2706.
[2]	CHENG Bei, WU Tao. Effects of Vitamin D on Reproductive Performance of Sows,Growth Performance of Piglets and Their Fecal Microbiota Structure [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4025-4034.
[3]	LI Huihui, ZHANG Meimei, YUAN Na, ZHANG Longchao, WANG Lixian, SHI Lijun. Study on the Changes in Jejunum Transcriptomics of Yorkshire Piglets Infected with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4141-4149.
[4]	CAI Yanzi, YUAN Ziwen, ZHANG Xingping, ZHANG Fengyu, ZHU Yamei, SHI Yan, JIANG Lidong, HUA Yongli, WEI Yanming. Study on Mechanism of Codonopsis pilosula-Glycyrrhiza Extract in Enhancing Immune Functions of Weaned Piglets Based on Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4279-4291.
[5]	WANG Yuan, WANG Wenwen, AN Xiaoping, QI Jingwei. Effects of Dietary Fermented Soybean Meal Replacement on Growth Performance, Digestive Enzyme,and Microbita Composition in Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 115-124.
[6]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[7]	WANG Manli, TAN Zihan, WANG Shuaijie, LI Qian, ZENG Yitong, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effects of Quercetin on Growth Performance, Blood Biochemical Indexes and Intestinal Absorption and Barrier Function in Piglets Infected with PEDV [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3411-3418.
[8]	CHEN Xueying, WANG Huisheng, WU Jie, DENG Bo, DIAO Xinping. Effects of Dietary Bacillus licheniformis and Bacillus amyloliquefaciens on Growth Performance and Blood Indicators of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2547-2556.
[9]	ZHANG Rui, ZHOU Weiqiang, MA Yue, WU Jianping, LEI Zhaomin. Effects of Oregano Essential Oil and Colostrum Complex on Growth Performance, Serum Indicators Related Antioxidant and Immune in Opportunity Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1314-1321.
[10]	WANG Chaopu, MA Xianyong, TIAN Zhimei, YU Miao, LIU Zhichang, DENG Dun, RONG Ting, TI Junling, SONG Min, ZHANG Huihua. Effects of Compound Chinese Medicine Fermentation Powder on Growth Performance,Nutrient Apparent Digestibility,Antioxidant Function and Immune Function of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1322-1331.
[11]	YANG Zhiwei, LI Zhixing, HUANG Tao, HAO Kexing, WANG Jing, HU Guangdong. Effects of Early Weaning and Transfer on Behavior, Growth Performance and Blood Indexes of Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 904-912.
[12]	BAI Peitian, KONG Jiamei, PEI Ting, CHENG Zhixue, REN Yuhong. Effects of Compound Microecological Preparation on Growth Performance, Immune Function and Caecal Flora Structure of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 942-952.
[13]	TIAN Zhimei, LU Huijie, DENG Dun, CUI Yiyan, LIU Zhichang, LI Jiazhou, MA Xianyong. Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2045-2055.
[14]	WANG Qian, WANG Manli, NING Nan, WANG Shuaijie, TAN Zihan, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effect of N-acetylcysteine on Immune Response and Intestinal Mucosal Energy Status in Piglets Challenged with Lipopolysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3996-4003.
[15]	SI Jinglei, HUANG Ye, CHEN Kuirong, LYU Dongling, CHENG Feng, TIAN Weilong, LIU Xiaoxiao, LIANG Liang, LI Yueyue, FENG Lingli, GAO Jiuyu, MO Jiayuan, LIANG Jing, LAN Ganqiu. Evaluating of the Association Between Early-stage Weight and Gut Microbiota in Duroc Pigs Using 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 190-199.