罗伊氏乳酸杆菌LR1对猪蛋白消化酶基因表达、肌肉抗氧化指标及氨基酸组成的影响

doi:10.16431/j.cnki.1671-7236.2021.06.017

Abstract

Abstract: This experiment was conducted to study effects of long-term Lactobacillus reuteri 1 (LR1) on the expression of enzymes involved in protein digestion in pancreas and stomach,antioxidant capacity and amino acid profile of meat in pigs.A total of 144 cross-bred (Duroc×Landrace×Yorkshire) weaned piglets (average body weight 6.49 kg±0.04 kg) at 21 days were randomly assigned to 3 groups.The diet in control groups was basal diet,the diet in antibiotic group was basal diet supplemented with antibiotics (100 mg/kg olaquindox plus 75 mg/kg aureomycin for weaned piglets,and 75 mg/kg aureomycin for growing and finishing pigs),and the diet in LR1 group was basal diet supplemented with 5×10¹⁰ CFU/kg LR1.The experiment lasted for 175 days.The results showed as follows:① Antibiotics decreased the expression of elastase in pancreas compared to control (P<0.05),LR1 increased pepsinogen A in antrum,cardia and fundus of stomach in pigs compared to control and antibiotics (P<0.05).② Compared to control group,antibiotics increased activities of total axtioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) in longissimus dorsis muscle (LDM) (P<0.05).LR1 did not affect malondialdehyde (MDA) content,activities of T-AOC、SOD and glutathione peroxidase (GSH-Px)in LDM of pigs (P>0.05).③ Both antibiotics and LR1 did not affect significantly total amino acids profile following hydrolysis (P>0.05).Antibiotics decreased free methionine (bitter amino acids),total content of sweet amino acids and free amino acids compared to chilled fresh pork at 24 h in contron group (P<0.05),and LR1 decreased methionine (P<0.05).Antibiotics and LR1 decreased the content of sweet,bitter,essential amino acids and total free amino acids (P<0.05),additionally,antibiotics decreased the content of flavor amino acids of chilled fresh pork at 48 h (P<0.05).In conclusion,long-term LR1 improved digestive function of protein in stomach and flavor of chilled fresh pork at 24 h of pigs.Long-term antibiotics decreased digestive function of pancreas,improved antioxidant capacity of muscle,but decreased flavor of chilled fresh pork at 24 h and 48 h of pigs.

Key words: Lactobacillus reuteri; pigs; digestive enzymes; antioxidant capacity; amino acids

CLC Number:

S816.7

TIAN Zhimei, LU Huijie, DENG Dun, CUI Yiyan, LIU Zhichang, LI Jiazhou, MA Xianyong. Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2045-2055.

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Effect of Lactobacillus reuteri 1 on the Gene Expression of Protein Digestion-related Enzymes,Antioxidant Indexes and Amino Acids Composition of Muscle in Pigs

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