大白猪仔猪感染流行性腹泻病毒后空肠转录组学变化研究

doi:10.16431/j.cnki.1671-7236.2023.10.028

Abstract

Abstract: 【Objective】 The widespread prevalence of porcine epidemic diarrhea (PED) has caused serious losses for pig farms.Existing vaccines provided very limited protection against mutated strains and the effective immune-related molecular markers screened from a host perspective were lacking.This study was aimed to identify differentially expressed genes (DEGs) in jejunum of Yorkshire piglets infected with Porcine epidemic diarrhea virus (PEDV),which would lay the foundation for research on the pathogenic mechanism of PEDV.【Method】 Eight 0-day-old Yorkshire piglets without colostrum were selected and rasied by milk powder until 3 days of age,four of them were attacked by PEDV as the infected group,and the other four piglets belong to control group.The jejunum were collected from the eight piglets died after infection and alive in control group for transcriptome sequencing.The genes associated with viral replication and organismal immune response were identified by differential analysis,and the GO and KEGG functional annotation were carried out for DEGs.In addition,five DEGs was randomly selected to perform Real-time quantitative PCR validation.【Result】 In total,16 256 protein coding genes (PCGs) were identified by quality control and comparative analysis of sequencing data,and 1 328 DEGs were detected through the differential analysis,of which 629 were up-regulated and 699 were down-regulated.GO function and KEGG pathway enrichment analysis revealed that up-regulated DEGs were significantly involved in 106 GO terms and 36 KEGG pathways,that were mainly associated with organismal systems and environmental signalling processes.The down-regulated DEGs were significantly involved in 54 GO terms and 23 KEGG pathways,that were mainly associated with metabolic responses.Real-time quantitative PCR results showed that the expression trends of CCND2,CREBBP,EGFR,MCL1 and PIK3CB genes were consistent with the transcriptome sequencing results.【Conclusion】 In this study,1 328 DEGs were identified,of which 629 were up-regulated and 699 were down-regulated.The up-regulated DEGs genes were mainly involved in environmental information processing and organismal system pathways,while the down-regulated genes were mainly involved in metabolic pathways,providing a theoretical basis for investigating the molecular mechanism of PEDV pathogenesis.

Key words: Yorkshire piglets; Porcine epidemic diarrhea virus (PEDV); transcriptome; functional enrichment

CLC Number:

S852.65⁺9.6

LI Huihui, ZHANG Meimei, YUAN Na, ZHANG Longchao, WANG Lixian, SHI Lijun. Study on the Changes in Jejunum Transcriptomics of Yorkshire Piglets Infected with Porcine Epidemic Diarrhea Virus[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4141-4149.

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Study on the Changes in Jejunum Transcriptomics of Yorkshire Piglets Infected with Porcine Epidemic Diarrhea Virus

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