单宁酸对猪流行性腹泻病毒增殖的体外抑制作用

doi:10.16431/j.cnki.1671-7236.2023.10.029

Abstract

Abstract: 【Objective】 This study was aimed to explore the effect of tannic acid on the replication of Porcine epidemic diarrhea virus (PEDV),and provide a new reference for clinical treatment of PEDV infection.【Method】 The toxicity of tannic acid on Vero and LLC-PK1 cells were assessed by detecting the effects of different concentrations of tannic acid on cell activity.The effect of tannic acid on the replication of PEDV YN13 strain was detected through Real-time quantitative PCR and indirect immunofluorescence assay.The effect of tannic acid on PEDV replication was tested by replacing the PEDV YN13 strain with the PEDV DR13-GFP strain and the Vero cell line to the LLC-PK1 cell line. The tannic acid was added at different time points of virus infection,and the influence of tannic acid on PEDV replication were detected by Real-time quantitative PCR,Western blotting and 50% tissue culture infectious dose (TCID₅₀),respectively.The extracellular effect of tannic acid on PEDV 3C-like proteases activity was examined by fluorescence resonance energy transfer test.【Result】 Low concentrations of tannic acid was almost nontoxic to the cells.Tannic acid could inhibit the replication of PEDV YN13 strain in a concentration dependent manner,all concentrations of tannic acid could inhibit PEDV replication after changing PEDV strain and cell lines.Tannic acid could inhibit PEDV infection by inhibiting PEDV adsorption or invasion process and viral early replication,and also inhibit PEDV 3C-like protease activity in a concentration dependent manner.【Conclusion】 Tannic acid could inhibit PEDV infection in vitro by affecting the viral adsorption or invasion process,and it might also inhibit the viral replication process by inhibiting the viral 3C-like protease.

Key words: tannic acid; Porcine epidemic diarrhea virus (PEDV); 3C-like protease; antiviral

CLC Number:

S816.44

ZENG Yitong, ZHU Liangyun, TONG Qingfang, HUANG Yizhi, LI Zhonghua, WU Tao, HOU Yongqing. Inhibition of Tannic Acid on Proliferation of Porcine Epidemic Diarrhea Virus in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4150-4159.

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Inhibition of Tannic Acid on Proliferation of Porcine Epidemic Diarrhea Virus in vitro

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