Abstract: 【Objective】 The experiment was to investigate the immunoregulatory effect of the compound Chinese mdicine preparations extracted from Cordyceps militaris,Gantinoderma lucidum,Astragalus membranaceus,Schisandra chinensis,Codonopsis pilosula,Angelica sinensis and Poria Cocos on H22 tumor-bearing mice.【Method】 The model of H22 tumor-bearing was established,and the mice were randomly divided into model group,high (250 mg/(kg·d)),medium (50 mg/(kg·d)) and low (10 mg/(kg·d)) dose groups of compound Chinese medicine preparation,each dose group was administrated intragastric once a day,and the model group was administrated intragastric with the same volume of normal saline.The experiment period was 3 weeks.The normal group was also set up for routine feeding.Whole anticoagulant blood was collected after the last administration,and the number of blood cells,the activity of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) and cytokines in serum were detected.Peripheral blood CD4⁺/CD8⁺ T cell subsets were detected by flow cytometry.The immune activity of tumor-bearing mice was investigated by spleen lymphocyte transformation test and carbon clearance test.【Result】 Compared with normal group,the number of white blood cells (WBC),platelets (PLT) and lymphocytes (W-SCR) in the blood of the model group was extremely significantly or significantly decreased (P＜0.01 or P＜0.05),the contents of interleukin-2 (IL-2),IL-6 and TNF-α,activities of SOD,Caspase-3 and concentration of cytochrome C (Cyt C) were extremely significantly or significantly decreased (P＜0.01 or P＜0.05).Compared with model group,the contents of IL-2,IL-6 and TNF-α in serum and the activities of SOD and Caspase-3 in high,medium and low dose groups were extremely significantly or significantly increased (P ＜ 0.01 or P ＜ 0.05),MDA content was extremely significantly or significantly decreased (P＜0.01 or P＜0.05).The amount of WBC and PLT in the medium dose group was extremely significantly or significantly increased (P＜0.01 or P＜0.05).The number of PLT in high dose group was extremely significantly increased (P＜0.01), and the concentration of Cyt C was significantly increased (P＜0.05).Flow cytometry showed that the number of T lymphocyte CD4⁺ and CD8⁺ subsets in model group was significantly decreased compared with that in normal group (P＜0.05).Compared with model group,the number of T lymphocyte CD4⁺ and CD8⁺ subsets in high and medium dose groups was significantly increased (P＜0.05),and the stimulation index,median hemolysis value and phagocytosis index of mice in high and medium dose groups were extremely significantly or significantly increased (P＜0.01 or P＜0.05).【Conclusion】 The compound Chinese medicine preparation could improve the immune activity of H22 tumor-bearing mice,and then promote the apoptosis of liver cancer cells.

Key words: compound Chinese medicine preparations; tumor-bearing mice; immunoregulation; liver cancer