太子参多糖对环磷酰胺所致肠道黏膜损伤小鼠SIgA、IL-2、IL-6含量的影响

doi:10.16431/j.cnki.1671-7236.2015.05.023

Abstract

Abstract: Effects of Pseudostellaria polysaccharides on the contents of intestinal mucosal SIgA, IL-2 and IL-6 in mice were reported in this paper.36 Kunming male mice with 18 to 22 g were randomly divided into six groups, blank control group, Pseudostellaria polysaccharides control group, cyclophosphamide (CY) model group, and Pseudostellaria polysaccharides low, middle, high dose groups.Each mouse in Pseudostellaria polysaccharides control group and Pseudostellaria polysaccharides low, middle, high dose groups was respectively treated with polysaccharides at the dose of 400, 100, 200 and 400 mg/kg body weight by intragastrical treatment for 19 continuous days, CY was given to each mouse in CY model group and Pseudostellaria polysaccharides low, middle, high dose groups by intraperitoneal injection with 100 mg/kg body weight in the 20th day, duodenum and ileum samples were collected from mice after 24 h, and SIgA, IL-2 and IL-6 contents were determined.The results showed that SIgA contents in duodenum were significantly increased (P<0.05) in Pseudostellaria polysaccharides middle and high dose groups compared to CY model group, SIgA contents in ileum were extremely significantly increased (P<0.01) in Pseudostellaria polysaccharides high dose group compared to CY model group.IL-2 contents in duodenum and ileum were significantly increased (P<0.05), IL-6 contents in duodenum were extremely significantly increased (P<0.01), IL-6 contents in ileum were significantly increased (P<0.05) in Pseudostellaria polysaccharides high dose group compared to CY model group.It was concluded that Pseudostellaria polysaccharide could antagonize intestinal mucosal injury caused by CY.

Key words: Pseudostellaria polysaccharides; cyclophosphamide (CY); immunoregulation; SIgA; IL-2; IL-6

CLC Number:

S853.7

YAN Sheng-ze, MA Yu-fang, LIAO Lv-yan, HUANG Yi-fan. Effect of Pseudostellaria Polysaccharides on SIgA,IL-2 and IL-6 Contents of Intestinal Mucosal Injured Mice Caused by Cyclophosphamide[J]. , 2015, 42(5): 1187-1192.

References

[1] 国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社, 2010.
[2] 汪剑飞.太子参药理研究新进展[J].实用药物与临床, 2013, 4:333-334.
[3] 黄文哲, 柳燕, 秦民坚, 等.太子参提取物对小鼠免疫功能的影响[J].现代屮药研究与实践, 2005, 19(6):35-37.
[4] 龚祝南, 戴岳, 马辉, 等.8个不同产地太子参对脾虚及免疫功能的影响[J].中药材, 2001, 24(4):281-282.
[5] Pang W, Lin S, Dai Q, et al.Antitussive activity of Pseudostellaria heteropliylla (Miq.) Pax extracts and improvement in lung function via adjustment of multi-cytokine levels[J].Molecules, 2011, 16(4):3360-3370.
[6] 蔡晶, 李孝栋, 陈旭征, 等.太子参多糖粗提物对小鼠免疫功能的影响[J].福建中医学院学报, 2005, 15(3):33-35.
[7] 袁逸铭, 高湘, 许爱霞, 等.太子参醇提物的抗脂质氧化作用[J].中国临床药理学与治疗学, 2005, 10(1):83-86.
[8] 张振明, 葛斌, 许爱霞, 等.太子参醇提物对大鼠组织和红细胞的抗氧化活性[J].第四军医大学学报, 2005, 26(22):2062-2064.
[9] 张振明, 许爱霞, 葛斌, 等.太子参水提物的抗氧化活性[J].中国医院药学杂志, 2006, 26(2):147-149.
[10] 沈祥春, 陶玲, 俞斌, 等.太子参对心肌梗死后慢性心衰大鼠的保护作用及对基质金属蛋白酶的影响[J].贵州科学, 2007, 25(S1):407-415.
[11] 沈祥春, 陶玲, 彭佼, 等.太子参对心肌梗死后慢性心衰大鼠心功能与基质金属蛋白酶表达的影响[J].中国病理生理杂志, 2008, 24(9):1694-1698.
[12] 沈祥春, 陶玲, 俞斌, 等.太子参对急性心肌梗死心力衰竭大鼠心肌重构的作用[J].贵阳医学院学报, 2008, 33(6):600-603.
[13] 贾玉海.常用中药八百味精要[M].第2版.北京:学苑出版社, 2001.
[14] 陈一, 钟正贤, 黄风桥, 等.蛤蚧的抗应激和免疫增强作用[J].中草药, 1985, 16(5):33.
[15] 刘训红, 陈彬, 王玉玺.太子参总皂苷药理作用的初步研究[J].江苏药学临床研究, 2000, 8(3):6-8.
[16] 徐宝林, 孙健, 杨锋, 等.补益药对细胞免疫功能影响的实验研究[J].浙江中医杂志, 1996, 31(5):219-220.
[17] 李越重.药物微生物技术[M]北京:化学工业出版社, 2004.
[18] 武金宝, 王继德, 张亚历.肠黏膜屏障研究进展[J].世界华人消化杂志, 2003, 11(5):619-623.
[19] 华素.穿心莲提取物的胃肠道吸收研究[D].开封:河南大学, 2013.
[20] 李相玲, 白莎莎, 周联, 等.环磷酰胺和地塞米松对小鼠肠道黏膜免疫抑制的比较[J].中国免疫学杂志, 2013, 1:39-42.
[21] 姜帆.五味子多糖的提取纯化及其对肠道免疫功能的影响[D].哈尔滨:东北农业大学, 2013.
[22] 胡庭俊, 陈灵然, 程富胜, 等.威麻多糖对小鼠血清中三种细胞因子水平的影响[J].中国兽医科技, 2005, 35(8):653-656.
[23] 曹露.鱿鱼墨多糖对环磷酰胺所致小鼠肠道黏膜上皮细胞损伤的保护作用研究[D].青岛:中国海洋大学, 2013.
[24] 吴志坚, 张永学.Radioimmunoassay of antiarrhythmic peptide and its application[J].华中科技大学学报(医学英德文版), 2003, 1:44-47.
[25] 卢小卓, 林之瑜, 陈怡霓.化学发光法、放免法检测血清TGA、TPoAb的比较[J].放射免疫学杂志, 2001, 1:20-22.
[26] 贾玉臣, 陈庆森.生物活性肽对肠黏膜免疫调节作用的研究进展[J].食品科学, 2009, 30(21):409-414.
[27] 王庭祥, 王庭欣, 刘峥颢, 等.海带多糖对小鼠肠黏膜组织SIgA的影响[J].安徽农业科学, 2009, 12:5515.
[28] 姬云丽, 凌虹, 于新慧, 等.当归补血汤多糖对隐孢子虫感染小鼠肠道局部免疫功能的影响[J].牡丹江医学院学报, 2007, 6:6-8.
[29] 胡婕伦.大粒车前子多糖体内外消化与酵解特征体系构建及其促进肠道健康的作用[D].江西:南昌大学, 2014.

Effect of Pseudostellaria Polysaccharides on SIgA,IL-2 and IL-6 Contents of Intestinal Mucosal Injured Mice Caused by Cyclophosphamide

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[2]	WANG Lu, HU Panpan, CHENG Jia, SUN Panpan, SUN Na, FAN Kuohai, YIN Wei, LI Hongquan, SUN Yaogui. Effect of Chuanwang Xiaoyan Powder on LPS Induced Acute Lung Injury in Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1568-1576.
[3]	GUO Zimeng, WANG Fuzheng, WANG Chengzhi, DU Peng, ZHANG Mingyue, LI Zeyao, SU Xiaochen, WU Gaofeng, YANG Jiancheng. Effect of Taurine Additive Package on the Immune Function of Immunosuppressed Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4635-4645.
[4]	LIU Sheng, ZHANG Jianan, JIA Ning, FANG Mei, DENG Kang, HUANG Weikuan. Analysis of Active Components of Total Flavonoids from the Seeds of Ammopiptanthus mongolicus and Its Effects on Immune Function in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2726-2736.
[5]	JIANG Xiaowen, WANG Xuewei, YU Wenhui, ZHANG Xin, HOU Yaojie, HAO Jingyou. Study on Immunoregulation of Radix Isatidis Ultrafine Powder in Rats [J]. , 2019, 46(7): 2159-2166.
[6]	LIU Kun, MAO Wei, LI Tingting, LIU Bo, GUAN Hong, PEI Le, CAO Jinshan. Effect of Escherichia coli and Staphylococcus aureus on Expression of Cytokine and Damage Degree of Dairy Cows Endometrium Tissue [J]. , 2019, 46(6): 1841-1848.
[7]	LIU Meifen, LI Qinnan, MA Zhenyuan, WU Zhiming, BAN Fuguo, YAN Ruoqian, WANG Huajun. Immunopotentiation Effect of Recombinant Chicken Interleukin-6/2 Fusion Protein on Newcastle Disease Virus (LaSota) Live Vaccine [J]. , 2019, 46(2): 590-599.
[8]	YAO Lili, BAI Lin, TAN Ying, SUN Jiaqi, SHI Dayou, GUO Shining, LIU Cui. Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3084-3094.
[9]	QIU Shulei, YANG Haifeng, CHEN Xiaolan, LIU Yunzhen, GUO Changming, YAO Jing, MENG Ting, ZHANG Yixin, WU Caihong. The Antagonistic Effect of Selenizing Garlic Polysaccharide on Immunosuppressed Chicken Induced by Cyclophosphamide [J]. , 2018, 45(10): 2885-2893.
[10]	WANG Pei, CHEN Yuan-yuan, HUO Jin-long, HUO Hai-long, WANG Shu-yan, PAN Wei-rong, CHENG Wen-min, ZHA Xing-qin, ZENG Yang-zhi. Cloning,Bioinformatics and Tissue Expression Analysis of IL-6 Gene in Banna Minipig Inbred Line [J]. , 2015, 42(8): 1925-1934.
[11]	YANG Li, ZHANG Li, QI Ya-yin, SHEN Wen, LI Yan, ZHANG Lu-an. Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep [J]. , 2015, 42(4): 890-895.
[12]	ZHANG Mei-hua, LI Jia, MENG Le, ZHOU Shuang-hai, JIANG Dai-xun, CHEN Wu. Study on Cyclic AMP Phosphodiesterase in Polymorphonuclear Neutrophils and cAMP,IL-6,TNF-α in Plasma of Porcine Respiratory Disease Complex and the Therapeutic Effect of Chinese Herbal Medicine [J]. , 2014, 41(9): 132-135.
[13]	LU Guo-min, SUN Hu-zhi, REN Hui-ying, ZHANG Can. Research Progress on Antibiotic Alternatives and its Mechanism of Action [J]. , 2014, 41(12): 276-281.
[14]	YANG Yu-hang, WANG Wen-yu, LIU Hong-na, LIU Dong-xu, SHI Kun, LI Jian-ming, DU Rui. Research Progress on the Detection Method of the Cell Vitality and Cell Immunity [J]. , 2013, 40(6): 142-145.
[15]	FAN Kuo-hai, LI Juan-juan, ZHANG Bei, LI Hong-quan. Effects of Astragalus Heteropolysaccharides on the Expression of IL-6 and PR-39 of Piglets [J]. , 2013, 40(2): 1-5.