冷应激对湖羊血清因子及热休克蛋白 70 mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2015.04.018

Abstract

Abstract: This experiment was to study the effect of cold stress on the immune system and heat stress proteins 70 (Hsp70) in Hu sheep. Using Real-time fluorescence quantitative PCR and ELISA, the expression quantity of Hsp70 mRNA in Hu sheep's liver, lung, spleen, lymph node tissue and concentrations of IL-4, IL-2 in serum were detected respectively before and after cold stress. The results showed that the Hsp70 mRNA expression quantity in liver, lung and spleen tissues of Hu sheep was extremely significantly increased after cold stress compared with before (P<0.01), and the expression of the liver was particularly significant. After cold stress, the IL-2, IL-4 concentrations in Hu sheep serum showed a decreasing trend, especially IL-4 concentration decreased extremely significantly (P<0.01). The results indicated that enhanced expression of Hsp70 mRNA after cold stress in Hu sheep tissues could improve the animal's function of self-protection and enhance resistance to adverse external stimulate, but the decreases in cytokines IL-2 and IL-4 concentrations indicated that cold stress suppressed the body's immune system.

Key words: cold stress; Hsp70; IL-2; IL-4; Hu sheep

CLC Number:

S826

YANG Li, ZHANG Li, QI Ya-yin, SHEN Wen, LI Yan, ZHANG Lu-an. Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep[J]. , 2015, 42(4): 890-895.

References

[1] 姜冬梅,李士泽,杨焕民,等.冷应激家兔血压与血清细胞因子的变化及其相互关系[J].环境与健康杂志,2007,24(8):19-22.
[2] 陈萍,杨焕民,李士泽,等.急性冷暴露对仔猪血浆中IL-2、IL-6、ACTH和皮质醇水平的影响[J].应用与环境生物学报,2009,15(1):91-94.
[3] 郭爽,杨焕民,郭景茹,等.不同强度冷刺激对大鼠血清中炎症相关细胞因子的影响[J].畜牧与兽医,2012,44(s1):310.
[4] 郭景茹,杨焕民,王忠伟,等.冷应激对畜禽免疫系统功能影响的研究进展[J].环境与健康杂志,2010,27(7):649-651.
[5] 周瑞进,郭景茹,宿甲子,等.急性冷暴露对仔猪PBMC中HSP70和血浆抗炎性细胞因子IL-4、IL-10的影响[J].中国兽医学报,2010,30(5):677-680.
[6] Huang L,Mivechi N F,Moskophidis D.Insights into regulation and function of the major stress-induced Hsp70 molecular chaperone in vivo:Analysis of mice with targeted gene disruption of the Hsp70.1 or Hsp70.3 gene[J].Molecular and Cellular Biology,2001,21(24):8575-8591.
[7] Vega V L,Rodríguez-Silva M,Frey T,et al.Hsp70 translocates into the plasma membrane after stress and is released into the extracellular environment in a membrane-associated form that activates macrophages[J].The Journal of Immunology,2008,180(6):4299-4307.
[8] Liew P K,Zulkifli I,Hair-Bejo M,et al.Effects of early age feed restriction and heat conditioning on heat shock protein 70 expression,resistance to infectious bursal disease,and growth in male broiler chickens subjected to heat stress[J].Poultry Science,2003,82(12):1879-1885.
[9] 王丹,仲庆振,孙泽威,等.热应激对肉鸡的影响及热休克蛋白70的研究进展[J].中国畜牧兽医,2011,38(1):51-54.
[10] 计红,吴永魁,胡仲明,等.不同强度冷应激下仔猪淋巴细胞HSP70 mRNA的转录规律[J].应用与环境生物学报,2007,13(4):507-509.
[11] 李士泽,任宝波,杨焕明,等.不同强度冷应激对大鼠肌肉、脾脏和肝脏中HSP70表达的影响[J].应用与环境生物学报,2006,12(2):235-238.
[12] Shim S B,Lee S H,Kim C K,et al.The effects of long-duration,low-temperature ground transportation on physiological and biochemical indicators of stress in mice[J].Lab Anim (NY),2008,37(3):121-126.
[13] 计红,杨焕明,李士泽,等.冷应激对仔猪血浆相关细胞因子和IgG水平的影响[J].应用与环境生物学报,2010,16(5):632-636.
[14] 尹剑春.不同负荷运动训练对大鼠血清白细胞介素2、皮质酮、下丘脑和血清β内啡肽及血浆T淋巴细胞亚群的影响[J].中国康复医学杂志,2006,21(7):593-595.
[15] 袁学军,牛静华,吴海燕,等.冷应激对伊褐红公雏鸡外周血淋巴细胞数目变化的影响[J].黑龙江八一农垦大学学报,2002,14(1):61-63.
[16] Hangalapura B N,Kaiser M G,Poel J J,et al.Cold stress equally enhances in vivo pro-inflammatory cytokine gene expression in chicken lines divergently selected for antibody responses[J].Dev Comp Immunol,2006,30(5):503-511.

Effect of Cold Stress on Serum Factor and Hsp70 mRNA in Hu Sheep

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