板蓝根超微粉对大鼠免疫功能调节作用的研究

doi:10.16431/j.cnki.1671-7236.2019.07.034

Abstract

Abstract:

In order to study the effects of ultrafine powder of Radix Isatidis on immune regulation in rats,cyclophosphamide was used to construct the model of immunodeficiency.80 SD rats were randomly divided into 4 groups:Model group,ordinary powder group,ultrafine powder group and blank control group with 20 rats in each group.40 mg/kg cyclophosphamide was injected intraperitoneally for 5 d.From 6th day,The blank control group and model group were given normal saline,and the other groups were given 5 g/kg ordinary powder or ultrafine powder.At 7th and 14th day,the blood samples and liver,spleen and thymus tissues were collected.The white blood cell count (WBC),organ indexes of spleen and thymus,C3b-R,ICR,phagocytosis indexes were detected.Real-time PCR was used to detect the transcription levels of TNF-α and IL-2 mRNA.The expression of TNF-ɑ and IL-2 protein were detected by Western blotting.The results showed that the WBC,body weight,thymus and spleen indexes in model group were significantly lower than those in the blank control group (P<0.05).On the 14th day,the percentage of macrophage phagocytosis of rat in ultrafine powder group reached 54.7%,the index of macrophage phagocytosis was 0.58.The C3b-R of rat at 14th day in ultrafine powder group was 9.44%,significantly higher than that of the ordinary power group (8.97%)(P<0.05).And the ICR of ultrafine powder group was 6.09%,lower than that of the ordinary power group (6.33%)(P>0.05).Compared with the ordinary powder group,the ultrafine powder of Radix Isatidis could increase the expression of TNF-ɑ and IL-2 in liver on the 14th day.In conlusion,ordinary powder and ultrafine powder of Radix Isatidis could both enhance the nonspecific immune function of rats,and ultrafine powder was superior to the ordinary powder.

Key words: Radix Isatidis ultrafine powder; Radixi Iatidis ordinary powder; SD rat; immunoregulation

CLC Number:

R283

JIANG Xiaowen, WANG Xuewei, YU Wenhui, ZHANG Xin, HOU Yaojie, HAO Jingyou. Study on Immunoregulation of Radix Isatidis Ultrafine Powder in Rats[J]. , 2019, 46(7): 2159-2166.

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Study on Immunoregulation of Radix Isatidis Ultrafine Powder in Rats

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