Abstract: 【Objective】 The purpose of this study was to investigate the effect of piceatannol (PIC) on liver injury induced by deoxynivalenol (DON) in mice.【Method】 Eighteen 5-week-old SPF grade C57BL/6J male mice with an initial weight of (19.3±0.8) g after 7 d of adaptive feeding were randomly divided into 3 groups based on the body weight：Control (CON),DON and PIC＋DON groups,with 6 mice in each group in a single cage.The experiment lasted for 14 d.Mice in CON group were given 0.2 mL PBS solution every day for 14 d.The mice in DON group were given 0.2 mL PBS solution continuously on days 1 to 7,and 0.2 mL DON (3 mg/kg BW) continuously on days 8 to 14.The mice in PIC+DON group were given 0.2 mL PIC (40 mg/kg BW) from days 1 to 7,and 0.2 mL DON (3 mg/kg BW) from days 8 to 14.Liver tissue and blood samples were collected after the experiment.The activities of glutathione peroxidase (GSH-Px) and catalase (CAT),total antioxidant capacity (T-AOC) and the content of malondialdehyde (MDA) in liver,the contents of total cholesterol (TC) and triglyceride (TG),and the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum were determined.The changes of liver histomorphology were observed by HE staining.Real-time quantitative PCR was used to detect the relative mRNA expression of interleukin-6 (IL-6),IL-1β,B-cell lymphomato-2-associated X protein (Bax),Caspase-3,Caspase-9,Caspase-1,microtubule associated protein 1 light chain 3β (LC3B),nuclear factor-κB (NF-κB),tumor necrosis factor-α (TNF-α),B-cell lymphoma-2 (Bcl-2) and nuclear factor E2-associated factor 2 (Nrf2) in liver.【Result】 Compared with CON group,the body weight of mice in DON group was significantly decreased (P＜0.05),and the liver index was significantly increased (P＜0.05).Compared with DON group,the body weight of mice in PIC＋DON group was significantly increased (P＜0.05),and the liver index was significantly decreased (P＜0.05).The pathological section results showed that the arrangement of liver cords was disordered,hepatocyte swelling and degeneration,endothelial cell shedding and inflammatory cells increased in DON group.After PIC treatment,the degree of lesion was significantly improved.Compared with CON group,GSH-Px,CAT activities and T-AOC of liver in DON group were significantly decreased (P＜0.05),MDA content of liver,TC and TG contents,AST and ALT activities of serum were significantly increased (P＜0.05).Compared with DON group,GSH-Px,CAT activities and T-AOC of liver in PIC＋DON group were significantly increased (＜0.05),MDA content of liver,TC and TG contents,AST and ALT activities of serum were significantly decreased (P＜0.05).Real-time quantitative PCR results showed that compared with CON group,the relative expressions of IL-6,Bax,Caspase-3, Caspase-9,Caspase-1,IL-1β,LC3B,NF-κB and TNF-α genes in DON group were significantly increased (P＜0.05)，and the relative expression of Bcl-2 and Nrf2 genes were significantly decreased (P＜0.05).Compared with DON group,the relative expressions of IL-6,Bax,Caspase-3, Caspase-9,Caspase-1,IL-1β,LC3B,NF-κB and TNF-α genes in PIC＋DON group were significantly decreased (P＜0.05)，and the relative expression of Bcl-2 and Nrf2 genes were significantly increased (P＜0.05).【Conclusion】 PIC could improve the liver injury induced by DON by increasing the activity of antioxidant enzymes and decreasing the expression of inflammatory factors.

Key words: piceatannol; deoxynivalenol; mice; liver injury