China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (9): 3833-3841.doi: 10.16431/j.cnki.1671-7236.2023.09.039

• Basic Veterinary Medicine • Previous Articles     Next Articles

Study on Protective Effects of Polypeptide LK-5 on Acute Liver Injury Induced by CCl4 in Mice

CHEN Jun1,4, LIAO Pengying2,3,5, ZHANG Xinrui2, HUANG Xiuhuan2, YANG Yujing2, LIU Huayu2   

  1. 1. Key Laboratory of TCM Neuro-metabolism and Immunopharmacology of Guangxi Education Department, Guangxi University of Chinese Medicine, Nanning 530200, China;
    2. College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, China;
    3. Key Laboratory of TCM Extraction and Purification and Quality Analysis, Nanning 530200, China;
    4. Teaching Experiment and Training Centre, Guangxi University of Chinese Medicine, Nanning 530200, China;
    5. National Demonstration Center for Experimental Traditional Chinese Pharmacology(Guangxi University of Chinese Medicine), Nanning 530200, China
  • Received:2023-02-16 Online:2023-09-05 Published:2023-08-24

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Abstract: 【Objective】 To investigate the protective effect and potential mechanism of polypeptide LK-5 (the amino acids sequence:LHMFK) on acute liver injury induced by carbon tetrachloride (CCl4) in mice.【Method】 72 mice were randomly divided into normal group,model group,biphenyl diester group (150 mg/kg),LK-5 low-dose,medium-dose and high-dose groups (30,60,120 mg/kg),with 12 mice in each group.Continuous administration of 10 mL/kg once a day for 10 days.Two hours after the last administration,each group (except for normal group) was intraperitoneally injected 0.1% CCl4 peanut oil solution to establish CCl4-induced acute liver injury model.16 h later,the serum levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT),total bile acid (TBA),and alkaline phosphatase (AKP),the superoxide dismutase (SOD),glutathioneperoxidase (GSH-Px),malondialdehyde (MDA),tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),IL-10,angiotensin 1-7 (Ang 1-7), and angiotensin Ⅱ (Ang Ⅱ) in liver were measured with kit methods.The pathological sections of liver were made,and the histological changes of liver were observed after hematoxylin and eosin (HE) staining.【Result】 Compared with normal group,the hepatocytes in model group showed obvious steatosis,and inflammatory cells were scattered around the blood vessels of liver lobule and in liver parenchyma,the ALT,AST,AKP activities and TBA level in serum were significantly increased,the SOD and GSH-Px activities in liver were significantly decreased,the MDA level was significantly increased (P＜0.05),indicating the acute liver damage model was established successfully,and the levels of TNF-α,IL-6 and Ang Ⅱ were significantly increased (P＜0.05).Compared with model group,the ALT and AST activities in serum were significantly decreased in LK-5 medium- and high-dose groups (P＜0.05),the serum TBA level and AKP activity in each group were significantly decreased (P＜0.05),the SOD and GSH-Px activities in liver were significantly increased in LK-5 high-dose group (P＜0.05),the MDA level in liver was significantly decreased in LK-5 medium- and high-dose groups (P＜0.05),the TNF-α level in liver was significantly decreased in high-dose group (P＜0.05), and the levels of IL-6 and Ang Ⅱ were significantly decreased in each group (P＜0.05).The pathological section results showed that inflammatory cells in each dose group of LK-5 decreased,while in the high-dose group,there was no infiltration of inflammatory cells around the hepatic lobular blood vessels and in the liver parenchyma.【Conclusion】 LK-5 showed protective effect on acute liver injury induced by CCl4 in mice in all dose groups,and 60, 120 mg/kg LK-5 had more significant effects. Its mechanism of action was related with the antioxidant,anti-inflammatory and regulation of renin-angiotensin system (RAS).

Key words: polypeptide LK-5; CCl4; acute liver injury; protective effects

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