桃金娘水提物抗肝脏纤维化作用的试验研究

doi:10.16431/j.cnki.1671-7236.2021.01.040

Abstract

Abstract: This experiment was conducted to study the protective effect of water extract of Rhodomyrtus tomentosa (Ait.) Hass on liver fibrosis rats induced by CCl₄ composited factor,and investigated its mechanism.72 SD mice were randomly divided to normal group,model group,colchicine group,low dose(1.5 mg/g),middle dose (3 mg/g) and high dose (6 mg/g) of water extract of Rhodomyrtus tomentosa (Ait.) Hass,12 mice for each group.Except for the normal group,rats in other groups were subcutaneously injected with 40% CCl₄ peanut oil solution at 1-4 weeks,0.3 mL/100 g body weight,once every other day for 4 weeks.At the same time,30% ethanol was gavaged as 1 mL/piece for 2-4 weeks,once every other day for 3 consecutive weeks.The rats in each group were given gavage at the 5th week,the normal control group and the model group were given normal saline,and the treatment group was given gavage at the corresponding dose,1 mL/100 g body weight,once a day for 4 weeks continuously.The rats in each group were still given gavage at the same time.At the end of the administration,serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity,total bilirubin (TBil) and alpha smooth muscle actin (α-SMA),transforming growth factor β (TGF-β) levels,malondialdehyde (MDA) content and superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) activity were detected.The changes of HE and Masson staining in liver tissues were observed.The results showed that water extract of Rhodomyrtus tomentosa (Ait.) Hass could significantly or extremely significantly reduce the activity/levels of AST,ALT,TBil,TGF-β and α-SMA in rats with liver fibrosis (P<0.05; P<0.01),increase the activity of SOD and GSH-Px in serum (P<0.05),reduce the content of MDA (P<0.05; P<0.01),and reduce the pathological damage degree of liver in rats with liver fibrosis.The above results showed that water extract of Rhodomyrtus tomentosa (Ait.) Hass had an obvious restorative effect on liver function of CCl4 composited factor induced liver fibrosis rats,enhanced its antioxidant capacity,alleviated hepatocyte injury,and had a good effect against liver fibrosis.

Key words: Rhodomyrtus tomentosa (Ait.) Hass; CCl₄; liver fibrosis; antioxidation

CLC Number:

S853.74

LIANG Liqing, HUANG Xin, CHEN Shaofeng, YANG Zengyan, SU Huazhen, LU Wei, WANG He. Experimental Studies on Anti-liver Fibrosis Effects of Water Extract of Rhodomyrtus tomentosa (Ait.) Hass[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 367-374.

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Experimental Studies on Anti-liver Fibrosis Effects of Water Extract of Rhodomyrtus tomentosa (Ait.) Hass

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