亲代三硝基丙酸诱导的氧化应激对胎鼠的影响

doi:10.16431/j.cnki.1671-7236.2021.06.040

Abstract

Abstract: This experiment was aimed to study the effects of oxidative stress on the offspring of mice.The male and female mice were injected 12.5 μg/g 3-nitropropionic acid (3-NPA) intraperitoneally,which were divided into normal male mice and normal female mice of control group (C),normal male and the 3-NPA treated female (FM),normal female and the 3-NPA treated male mice (MM) and 3-NPA treated male mice and the 3-NPA treated female mice mating group (DM) for 3 times.The number of progeny fetal mice was counted by the method of secting fetal mice.The heart,liver and lung of the progeny fetal mice were collected and the activities of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) of the progeny fetal mice were determined.The results showed that compared with the control group,the number of fetal mice in FM group was not significantly decreased (P>0.05),while the number of fetal mice in MM and DM groups was significantly decreased (P<0.05).The CAT activity in liver and lung of fetal mice in FM and DM groups was significantly increased (P<0.05).The CAT activity in liver and lung of fetal mice in MM group was increased,but the difference was not significant (P>0.05).CAT activity in heart increased in all groups,but there was no significant difference (P>0.05).T-AOC activity in liver,lung and heart was significantly increased (P<0.05),except for in lung in MM group (P>0.05).The activity of GSH-Px in fetal liver of DM group was significantly increased (P<0.05),and increased in FM and MM groups,but the difference was not significant (P>0.05).The activity of GSH-Px in lung of fetal mice in FM and DM groups was significantly increased (P<0.05).There was no significant difference in GSH-Px activity among all groups in heart (P>0.05).The activity of SOD in liver,lung and heart of fetal mice in DM group was significantly increased (P<0.05),and increased in liver,lung and heart in FM and MM groups,but the differences were not significant (P>0.05).Therefore,the oxidative stress mouse model of 3-NPA was successfully established in this study,and the oxidative stress of parental male mice had a greater effect on fetal mice.

Key words: fetal mice; organ; oxidative stress; 3-nitropropionic acid; antioxidation

CLC Number:

S814

GAO Jianhong, YU Jiatong, WANG Huirui, LIU Xu, GUO Yong, FANG Nanzhu, LIU Haixing. Effects of Oxidative Stress Induced by Parental 3-nitropropionic Acid on the Fetal Mice[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2255-2261.

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Effects of Oxidative Stress Induced by Parental 3-nitropropionic Acid on the Fetal Mice

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