China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (9): 4080-4091.doi: 10.16431/j.cnki.1671-7236.2024.09.036

• Basic Veterinary Medicine • Previous Articles    

Study on Allicin Alleviating the Liver Injury of Quail Caused by Fumonisin B1

LI Xinran1, ZHU Lingxin1, SONG Huanni1, ZHU Xueyan1, LI Ruobin1, LIU Yang2, CAO Changyu1   

  1. 1. College of Life Science and Engineering, Foshan University, Foshan 528225, China;
    2. Quality Control Technical Center (Foshan) of National Famous and Special Agricultural Products(CAQS-GAP-KZZX 043), College of Food Science and Engineering, Foshan University, Foshan 528225, China;
    3. Foshan University Veterinary Teaching Hospital, Foshan 528225, China
  • Received:2023-12-11 Published:2024-08-27

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Abstract: 【Objective】 The objective of this experiment was to explore the mechanism of allicin in alleviating liver injury caused by fumonisin B1 (FB1) in quail during egg-laying period, so as to provide experimental basis for the prevention of FB1-induced liver injury in quail, and reduce the economic loss caused by FB1 poisoning to the breeding industry. 【Method】 The Yellow-feathered female quails were used as test subjects, and 120 quails were randomly divided into 4 groups：Control group, allicin group (500 mg/kg allicin), fumonisin group (30 mg/kg FB1), and remission group (500 mg/kg allicin+30 mg/kg FB1), with 30 quails in each group.The histopathological and ultrastructural changes of liver in quails were observed, the indexes of liver function, antioxidant function and the expression of related inflammatory factors in quail were detected. 【Result】 The results of liver pathology and ultrastructure showed that allicin could effectively alleviate the pathological damage caused by FB1, such as the disappearance of mitochondrial cristae and the increase of collagen fibers.Compared with control group, the concentration of aspartate aminotransferase (AST) in serum of quails in fumonisin group was significantly increased (P＜0.05), the activitiy of catalase (CAT) and total antioxidant capacity (T-AOC) were significantly decreased (P＜0.05), and the malondialdehyde (MDA) content was significantly increased (P＜0.05).The expressions of interleukin-8 (IL-8), IL-6, IL-18, IL-12β, tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), related apoptosis factor ligand (FASL) and Toll-like receptor 4 (TLR4) genes were also significantly increased (P＜0.05).Compared with fumonisin group, the AST content in serum of quails in remission group was significantly decreased (P＜0.05), the activitiy of CAT and T-AOC were significantly increased (P＜0.05), and MDA content was decreased significantly (P＜0.05).The expressions of IL-8, IL-6, IL-18, IL-12β, TNF-α, COX-2, iNOS, FASL and TLR4 genes were also significantly decreased (P＜0.05). 【Conclusion】 Allicin could effectively alleviate FB1-induced liver injury in quails, and its mechanism was closely related to the anti-inflammatory and antioxidant effects of allicin.

Key words: quails; allicin; fumonisin B1; liver injury

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