刺糖对对乙酰氨基酚诱导的肝损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2017.07.040

Abstract

Abstract:

N-acetyl-para-aminophenol (APAP) overdose use induced liver damage.This study was aimed to evaluate the hepatoprotective effect of Saccharum alhagi against APAP-induced liver injury in mice. The aqueous extract of Saccharum alhagi was prepared, ant its ingredients were measured by phenol-sulfuric acid method and aluminium salt chromogenic method. The mice were randomly divided into seven groups,including normal control group,APAP model group,Saccharum alhagi (150, 300 and 600 mg/kg·BW)+APAP groups,positive control group and Saccharum alhagi control group. Administration once per day for 3 consecutive days,with 300 mg/(kg·BW) of APAP after 1 h from the last administration of Saccharum alhagi. 300 mg/(kg·BW) APAP were used to induce liver injury, and after 24 h from APAP challenge,the experimental animals were sacrificed to collect blood and liver tissue samples. The level of serum transaminase (ALT and AST) and the liver pathological changes were observed. The results indicated that the levels of serum AST and ALT were extremely significant lower in the Saccharum alhagi treated groups than the APAP treated group (P<0.01), and the pathological changes were significantly improved. This study showed that Saccharum alhagi could be an effective therapeutic source in APAP-induced liver injuries in mice.

Key words: Saccharum alhagi; APAP; liver injury

CLC Number:

S852

AILI·Aierken, KUERBANJIANG·Maimaitimin, JIANG Zhi-hui, ZHANG Xiao-ying, MIKEREMU·Shayibuzhati. Protective Effect of Saccharum alhagi Against N-acetyl-para-aminophenol induced Liver Injury in Mice[J]. , 2017, 44(7): 2185-2190.

References

[1] Lu L, Feng M, Gu J,et al. Restoration of intrahepatic regulatory T cells through MMP-9/13-dependent activation of TGF-P is critical for immune homeostasis following acute liver injury[J]. J Mol Cell Biol,2013,5(6):369-379.
[2] Jaeschke H,Xie Y C,McGill M R. Acetaminophen-induced liver injury:From animal models to humans[J]. J Clin Trans Hepatology,2014,2(3):153.
[3] Larson A M,Polson J,Fontana R J,et al. Acetaminophen-induced acute liver failure results of a United States multicenter prospective study[J]. Hepatology,2006,43(4):880-882.
[4] 汪倩,徐瑞娟,杨劲. 对乙酰氨基酚肝毒性机理及药物干预靶点[J].药学与临床研究,2011,19(3):247-252.
[5] Manthripragada A D,Zhou E H,Budnitz D S,et al. Characterization of acetaminophen overdose-related emergency department visits and hospitalizations in the United States[J].J Pharmacoepidem DR S,2008,20(8):819-826.
[6] Mcgill M R,Sharpe M,Williams C D,et al. The mechanism underlying acetaminophen-induced hepatotoxicity in humans and mice involves mitochondrial damage and nuclear DNA fragmentation[J].J Clin Investig, 2012,122(4):1574-1583.
[7] Aleksunes L M,Scheffer G L,Jakowski A B,et al. Coordinated expressionof multidrug resistance-associated proteins (Mrps) in mouse liver during toxicant-induced injury[J].J Toxicol Sci,2006,89(2):370-379.
[8] Lawrence R A,Burk R F.Glutathione peroxidase activity in selenium-deficient rat liver[J].Biochem Biophys Res Commun, 2012,425(3):503-509.
[9] 耿东升.维吾尔医药基本理论及其药物研究进展[J].中国民族医药杂志,2007,3(3):9-14.
[10] Ahmad N,Bibi Y,Saboon,et al. Traditional uses and pharmacological properties of Alhagi maurorum:A review[J].Asian Pac J Trop Dis,2015,5(11):856-861.
[11] 茹克娅木·沙地克. 维吾尔医常用药材学[M].乌鲁木齐:新疆科技卫生出版社,1993.
[12] Gulzar M,Muhammad A H, Farooq A, et al. Alhagi:A plant genus rich in bioactives for pharmaceuticals[J]. J Phytotherapy Research,2014,29(1):1-13.
[13] Dong Y, Huang J, Lin X, et al. Hepatoprotective effects of Yulangsan polysaccharide against isoniazid and rifampicin-induced liver injury in mice[J].J Ethnopharmacology,2014,152(1):201-206.
[14] 贾磊. 骆驼刺油脂活性成分及其保健功能研究[J]. 甘肃农业大学学报, 2008, 43(5):167-169.
[15] 程煜凤. 维药刺糖化学成分的基础研究[D].乌鲁木齐:新疆医科大学,2010.
[16] Cover C,Mansouri A, Knight T R, et al. Peroxynitrite-induced mitochondrial and endonuclease-mediated nuclear DNA damage in acetaminophen hepatotoxicity[J]. J Pharm ExperTherape,2005,315(2):879-887.
[17] 刘霞飞,吴骁,杨方秀,等. 正柴胡饮对对乙酰氨基酚所致小鼠急性肝损伤的保护作用[J]. 中国药理学与毒理学杂志, 2017,31(1):101-111.
[18] Gum S I, Cho M K. Korean red ginseng extract prevents APAP-induced hepatotoxicity through metabolic enzyme regulation:The role of ginsenoside Rg3 a protopanaxadiol[J]. Liver Inter,2012,33(7):1071-1084.
[19] Yousef M I,Omar S A,Elguendi M I,et al. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat[J]. Food Chem Toxicol,2010,48(11):3246-3261.
[20] Xie W,Chen C,Jiang Z,et al. Apocynum venetum attenuates acetaminophen-induced liver injury in mice[J]. Am J Chin Med,2015,43(3):457.

Protective Effect of Saccharum alhagi Against N-acetyl-para-aminophenol induced Liver Injury in Mice

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 10

Recommended Articles

Metrics

Comments

[1]	AN Zhixia, PAN Yangyang, FAN Biyue, MA Xiaoyan, YE Dehe, ZHANG Zhijie, QIU Shantong, YAO Yale, WANG Meng. Study on the Effect of Forsythia suspensa Leaf Extract on Enrofloxacin-induced Liver Injury Through AMPK Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2347-2361.
[2]	ZHAO Weijie, SUN Yaogui, SUN Panpan, SUN Na, YANG Huizhen, LI Hongquan. Study on Repair Effect and Mechanism of Nigella sativa Seed Extract on Alcoholic Liver Injury in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4928-4935.
[3]	MENG Jinyan, XU Baichang, SI Hongbin. Study on the Protective Effect of Puerarin on Acetaminophen-induced Acute Liver Injury [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4457-4465.
[4]	GENG Yumeng, LI Shuying, LU Chunyu, CUI Yuqing, SHI Wanyu, BAO Yongzhan. Study on the Molecular Mechanism of Salvia miltiorrhiza Polysaccharide in Inhibiting Florfenicol-induced Liver Injury in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1849-1858.
[5]	ZHAO Chendong, WANG Meng, ZHANG Hao, DAI Guonian, AN Zhixia, SHEN Yali, WANG Guirong. Protective Effect of Forsythiae suspensa Extract on Acetaminophen-induced Liver Injury in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3845-3854.
[6]	YANG Hao, JIN Sanjun, PANG Qian, FENG Xingjun. Research Progress on the Protective Mechanism and Application of Resveratrol to Common Liver Injury in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3528-3535.
[7]	HAO Sina, ZHUO Na, HAI Le, YI Li, MING Liang, JIRIMUTU. Protective Effect of Camel Urine on Liver Injury Induced by CCl₄ in Mice [J]. , 2019, 46(5): 1316-1322.
[8]	YE Wei-qing, DU Bing-wang, LI Dong-hua, LI Nai-bin. Establishment of Experimental Animal Models of Kirin Chicken (Chicken Feather Volume) Liver Injury Induced by Heat Stress [J]. , 2015, 42(3): 741-744.
[9]	LIU Hong-wei;ZHAO Li-qin;ZHANG Tian-tian;SHEN Ming-hao;LI Mu-ling. Protection Effect of Spiraea pubescens against Acute Alcohol-induced Liver Injury in Mice [J]. , 2012, 39(4): 166-168.
[10]	ZHAO Liang-zhong;SHI Wen-yan;JU Xiao-hong;LI Yan. The Therapeutic Effects of EA-PAOA on Liver Injury Induced by Concanavalin A in Kunming Mice [J]. , 2010, 37(3): 160-163.