阿司匹林丁香酚酯对大鼠的急性毒性研究

doi:10.16431/j.cnki.1671-7236.2017.07.039

Abstract

Abstract:

The acute toxicity study was carried out by intragastric administration in rats to evaluate the toxic characteristics of aspirin eugenol ester (AEE) and target organs and fully analyze the reason of death rats. The experiment was designed in accordance with the method provided by the Kärber arithmetic method. At first, 50 rats (half male and half female)were divided into 5 groups to calculate LD₁₀₀ and LD₀. According to the pre-test results, different doses of AEE (2.62,3.25,4.03,5.00,6.20,7.69 and 9.53 g/kg) and control group of 0.5% CMC-Na were orally administered to 80 rats, half male and half female, divided into 8 groups. After treatment, clinical signs of rats and the change of body weights were observed and recorded. The microscopic pathology of mild damages on organs was conducted. 14 days after adminstration, the death time and total deaths of rats were recorded, and the LD₅₀ and 95% confidence interual of AEE were calculated according to Kärber. Organs were fixed with 10% formaldehyde solution at the end of the experiment. The LD₅₀ of AEE in rats was 5.95 g/kg upon oral administration and its 95% confidence interval calculation was 5.30 to 6.68 g/kg. The death time of rats were centralized at 48 to 96 h after exposing to AEE more than 4.03 g/kg by the analysis of the cumulative mortality-time of AEE in each group. Significant decreases in body weights were noted after treatment of AEE for 3 days, after that body weights gradually grew and recovered at the 5th day. Remarkable damages on livers, kidney and gastrointestinal tract were found at AEE dosage of more than 5.00 g/kg. From the obtained value of LD₅₀>5.00 g/kg, AEE was classified as practically non-toxic compound according to WHO. The target organs of AEE were liver, kidney and gastrointestinal tract.

Key words: aspirin eugenol ester; acute toxicity; intragastric administration; rats

CLC Number:

S859.7

ZHAO Xiao-le, KONG Xiao-jun, MA Ning, YANG Ya-jun, LIU Xi-wang, SHEN Dong-shuai, LI Jian-yong. Acute Toxicity of Aspirin Eugenol Ester on Rats[J]. , 2017, 44(7): 2178-2184.

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Acute Toxicity of Aspirin Eugenol Ester on Rats

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