螺旋藻激酶对大鼠动脉粥样硬化的影响

doi:10.16431/j.cnki.1671-7236.2019.12.037

Abstract

Abstract: The study was aimed to observe the effects and preliminary mechanism of Spirulina kinase (SPK) on atherosclerosis (AS) model rats.60 SD rats were randomly divided into normal control group,model group,positive control group (simvastatin,0.005 g/kg),SPK low-dose,medium-dose and high-dose groups(80,160 and 320 U/kg).Except for normal control group,other groups were fed with high fat feed to establish the atherosclerosis model and were given relevant medicine intragastrically once a day for 12 weeks.The artery blood was collected and the content of total cholesterol (TC),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C) levels,contents of MDA,NO and SOD activity were measured,the atherosclerosis index (AI) was caculated.The change of thoracic aorta were observed by hematoxylin and eosin (HE) staining under microscope.The results showed that:Compared with normal control group,AI and MDA content were significantly increased (P<0.05),SOD activity and NO level were significantly decreased(P<0.05) in model group;Compared with the model group,MDA content was significantly decreased (P<0.05),while SOD activity and NO level were significantly increased (P<0.05) in SPK high-dose group.Pathological results showed that the model group had obvious atherosclerotic plaque formation.The formation of atherosclerotic plaque in SPK groups was significantly reduced,especially in the middle and high dose groups,there was no obvious difference comparing to the normal control group.In conclusion,SPK had obvious effect of preventing AS.Its mechanism might be related to the decrease of MDA and the increase of SOD activity and NO level.

Key words: Spirulina kinase (SPK); rats; atherosclerosis (AS); malondialdehyde; superoxide dismutase; nitric oxide

CLC Number:

R965

WANG Huijie, PANG Hui, WEI Jiao, WU Guo. Effects of Spirulina Kinase on Atherosclerosis Model Rats[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3768-3774.

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Effects of Spirulina Kinase on Atherosclerosis Model Rats

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