内脂素对脂多糖刺激的大鼠肝脏组织结构和髓过氧化物酶的影响

doi:10.16431/j.cnki.1671-7236.2015.01.018

Abstract

Abstract: The present study was aimed to investigate the effects of visfatin on the morphological structure and MPO in liver of LPS-induced rats.24 8-week-old Wistar rats were randomly divided into 4 groups including saline group, visfatin group, LPS group and visfatin+LPS group.Wistar rats in saline group and LPS group were both received NaCl solution injection each day, Wistar rats in visfatin group and visfatin+LPS group were both received the injection of visfatin each day.One week later, LPS was injected into Wistar rats in LPS group and visfatin+LPS group.All the Wistar rats were sacrificed 6 h after the injection of LPS.HE and immunohistochemical methods were used to observe the morphological structure and MPO in liver of each group.The present study showed that the macrophage increased and the expression level of MPO reduced significantly in visfatin group than saline group (P< 0.05).Compared with LPS group, in vifatin+LPS group, morphological of hepatocytes became disorder, the infiltration of inflammatory cells became more clear, the expression level of MPO reduced significantly (P< 0.05).The results suggested that visfatin could increase the number of macrophage and had a remarkable effect on MPO level in liver.

Key words: visfatin; LPS; Wistar rats; liver; MPO

CLC Number:

Q593^＋.1

YANG Zhi, GAO Min, XIAO Ke, WANG Wei, SONG Hui, PENG Ke-mei. Effect of Visfatin on Morphological Structure and MPO in Liver of LPS-induced Rats[J]. , 2015, 42(1): 118-123.

References

[1] Fukuhara A,Matsuda M,Nishizawa M,et al.Visfatin:A protein secreted by visceral fat that mimics the effects of insulin[J]. Science,2005,307(5708):426-430.
[2] Samal B,Sun Y,Stearns G,et al.Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor[J].Molecular and Cellular Biology,1994,14(2):1431-1437.
[3] 油红捷,闫韶飞,丁卫.脂肪因子 visfatin 的调节与功能多样性[J]. Progress in Biochemistry and Biophysics,2008,35(12):1351-1357.
[4] Moschen A R,Gerner R,Schroll A,et al.A key role for pre-B cell colony-enhancing factor in experimental hepatitis[J].Hepatology,2011,54(2):675-686.
[5] Moschen A R,Gerner R,Tilg H.Pre-B cell colony enhancing factor/NAMPT/visfatin in inflammation and obesity-related disorders[J].Current Pharmaceutical Design,2010,16(17):1913-1920.
[6] Liang W,Lindeman J H,Menke A L,et al.Metabolically induced liver inflammation leads to NASH and differs from LPS- or IL-1β-induced chronic inflammation[J].Laboratory Investigation,2014,94(5):491-502.
[7] Tilg H,Hotamisligil G S.Nonalcoholic fatty liver disease: Cytokine-adipokine interplay and regulation of insulin resistance[J]. Gastroenterology,2006,131(3):934-945.
[8] Lago F,Gómez R,Gómez-Reino J J,et al.Adipokines as novel modulators of lipid metabolism[J].Trends Biochem Sci,2009,34:500-510.
[9] Kukla M,Berdowska A,Gabriel A,et al.Association between hepatic angiogenesis and serum adipokine profile in non-obese chronic hepatitis C patients[J].Pol J Pathol,2011,62(4):218-228.
[10] 彭克美.动物组织学与胚胎学[M].北京: 高等教育出版社,2009.
[11] Curat C A,Wegner V,Sengenes C,et al.Macrophages in human visceral adipose tissue: Increased accumulation in obesity and a source of resistin and visfatin[J]. Diabetologia,2006,49:744-747.
[12] Moschen A R,Kaser A,Enrich B,et al.Visfatin,an adipocytokine with proinflammatory and immunomodulating properties[J].Journal of Immunology,2007,178:1748-1758.
[13] Xu H,Xu W,Chu Y,et al.Involvement of up-regulated CXC chemokine ligand 16/scavenger receptor that binds phosphatidylserine and oxidized lipoprotein in endotoxin-induced lethal liver injury via regulation of T-cell recruitment and adhesion[J].Infection and Immunity,2005,73:4007-4016.
[14] Raetz C R.Biochemistry of endotoxins[J].Annual Review of Biochemistry,1990,59:129-170.
[15] Cohen J.The immunopathogenesis of sepsis[J].Nature,2002,420:885-891.
[16] Kobayashi S,Nishihira J,Watanabe S.Prevention of lethal acute hepatic failure by antimacrophage migration inhibitory factor antibody in mice treated with Bacille Calmette-Guerin and lipopolysaccharide[J].Hepatology,1999,29:1752-1759.
[17] McGlothlin J R,Gao L,Lavoie T,et al.Molecular cloning and characterization of canine pre-B-cell colony-enhancing factor[J] Biochemical Genetics,2005,43:127-141.
[18] 林建敏.中国中性粒细胞MPO完全缺陷家系的分子机制研究[D].福州:福建医科大学,2013.
[19] Lau D,Baldus S.Myeloperoxidase and its contributory role in inflammatory vascular disease[J].Pharmacology & Therapeutics,2006,111:16-26.
[20] 胡涛,王加启,张养东,等.阴外动脉注射脂多糖对奶牛临床症状及白细胞的影响[J].中国畜牧兽医,2011,38(6):5-9.
[21] Li L,Bhatia M,Zhu Y Z,et al.Hydrogen sulfide is a novel mediator of lipopolysaccharide-induced inflammation in the mouse[J].FASEB Journal:Official Publication of the Federation of American Societies for Experimental Biology,2005,19:1196-1198.

Effect of Visfatin on Morphological Structure and MPO in Liver of LPS-induced Rats

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHONG Yueyun, YANG Shuzhan, CHEN Feiyue, LU Zhier, LI Bingxin, LI Wanyan, TIAN Yunbo, HUANG Yunmao, XU Danning, CAO Nan. Effects of LPS on Apoptosis of Goose Embryo-derived Hepatocytes ROS/p38/MAPK Signaling Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2224-2232.
[2]	FAN Yimeng, WEI Yuanyuan, WANG Huiru, GA yu, ZHANG Yannan, ZHAO Qingyu, HAO Zhihui. Potential Mechanisms of Chinese Veterinary Medicine Compound Rukang Granules in the Treatment of Dairy Cow Mastitis Based on Network Pharmacology and Molecular Docking Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2507-2517.
[3]	XUE Zhanmei, LIU Xuesong, ZHANG Yan, ZHANG Bei, MENG Weishan, ZHANG Guohua, WANG Yan, ZHONG Peng, QIU Jinghui, TANG Jilong, SHI Tongrui. Effect of Bacillus velezensis Fermentation on Extraction of Active Components from Traditional Chinese Medicine of Rukang Prescription [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2540-2549.
[4]	XU Jiahui, XIE Jiaxin, JIANG Chengkun, ZHANG Sen, YAO Hua, ZHANG Hua. Application of Three-dimensional Reconstruction and 3D Printing Technology in Beagle Dog Liver Model Construction [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2130-2138.
[5]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[6]	LIU Xin, CHEN Qun, QIU Yulang, LI Lin, HOU Guoxi, YAN Xiaogang, LI Zhonghe, CHEN Long, GAO Xingai, LIU Dongmei, ZHAI Di, YAN Qiuliang. Research Progress on the Production Mechanism and Control Technology of Chicken Manure Composting Nitrogen-containing and Sulfur-containing Waste Gases [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2166-2174.
[7]	GUO Hongwei, CAI Yongchao, FAN Chunguang, HAO Kaixuan, CHANG Juan, WANG Ping, LIU Chaoqi, YIN Qingqiang. Effect of Compound Biological Detoxifier on Aflatoxin B₁ Degradation Rate Under Artificial Gastrointestinal Fluid Condition [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1395-1403.
[8]	CHEN Jin, XIE Jinghao, HAN Yingqian, YANG Yanbin, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Effects of Euphorbiae humifusae Aqueous Extract in IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 704-712.
[9]	WU Tao, QI Yunxia, LIU Wei, ZHU Honglong, HUANG Dongwei, DING Haisheng, ZHAO Huiling, CHENG Guanglong, ZHAO Xiaowei. Effect of Colostrum and Milk Feeding on Hepatic Proteins of Calves Based on Proteomics Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 37-45.
[10]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[11]	ZENG Zhiqin, LOU Wenqi, XU Jingyi, MA Yehua, ZHANG Yongfu, REN Xiaoli, CHEN Shaokan, YAN Lei, GUO Gang, ZHANG Zhen, WANG Yachun. Analysis of Impact Factors and Phenotype Prediction for Transforming Progesterone Concentration in Chinese Holstein Cows During Lactation [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 186-197.
[12]	ZHU Xuedan, PAN Xueqing, MA Junyan, ZHOU Lele, ZHANG Shouquan. Research Progress on the Effects of Prostaglandin F_2α and Its Analogues on the Reproductive Performance of Mammal [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3072-3082.
[13]	SUN Yuelong, ZHANG Mengjie, DOU Jiahong, WANG Xiaoying, DAI Xiaofeng, LI Xiumei. Effects of Astragalus membranaceus (Fisch.) Bunge.on Piglets Flora Imbalance Diarrhea Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3200-3211.
[14]	REN Jianming, JI Peng, ZHANG Xiaosong, WEI Yanming, ZHU Xiaopeng, ZHU Yueming. Effects of Jianpi Xiaodao Chinese Herbal Compound on Growth Performance, Rumen Fermentation Parameters and Serum Indexes of 6-month-old Simmental Crossbred Cattles [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2593-2600.
[15]	XU Xiaoling, BAI Jiahua, XIAO Linli, QIN Yusheng, SONG Yuqing, LIU Yan. Effects of Compound Additives on the Quality and Conception Rate of Dairy Cows Thawed Sex Controlled Frozen Semen [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2654-2660.