丹参多糖抑制氟苯尼考致肉鸡肝脏损伤的分子机制研究

doi:10.16431/j.cnki.1671-7236.2021.05.037

Abstract

Abstract: This experiment was conducted to explore transcriptome targets and pathways of Salvia miltiorrhiza polysaccharides in alleviating liver injury induced by flufenicol in broilers.Thirty one-day-old broilers were randomly divided into three groups:blank control group (LA group) were fed tap water,florfenicol model group (LB group) was given tap water containing 0.15 g/L florfenicol,and Salvia miltiorrhiza polysaccharides treatment group (LC group) was given tap water containing 0.15 g/L florfenicol and 5 g/L Salvia miltiorrhiza polysaccharides tap water.The drug was administered continuously for 5 days from the age of 1 day.On day 6,three chickens were randomly selected from each group and killed,and their liver tissues were aseptically extracted.After RNA extraction,purification and library construction of liver tissues,the Illumina HiSeq libraries were sequenced using the Illumina HiSeq platform.Then the original offline data was filtered and the high-quality sequences obtained after filtering were aligned to the reference genome of the species.The expression level of each gene was calculated according to the comparison results.3 groups chicken liver digital gene expression patterns (DGE) library were built respectively,the DGE library of differentially expressed genes were compared,looking for the key genes with most significant difference in gene expression,and GO function annotation,KEGG pathway were analyzed.The results showed that 1 989 (495 up-regulated,1 494 down-regulated),1 278 (344 up-regulated,934 down-regulated) and 380 genes (165 up-regulated,215 down-regulated) were screened out in the comparison groups of LA vs LB,LA vs LC and LB vs LC,respectively.The GO functional annotations of LA vs LB,LA vs LC and LB vs LC comparison groups were mainly classified into two branches:biological process and cell composition.The results of the KEGG pathway analysis showed that differentially expressed genes of LA vs LB were mainly enriched in drug metabolism-cytochrome P450,extracellular matrix receptor interaction,cytokine-cytokine receptor interaction,metabolism of xenobiotics by cytochrome P450,glutathione metabolism,peroxidase body growth activated receptor (PPAR) signaling pathway,glycine,serine and threonine metabolism,alanine,aspartate and glutamate metabolism pathways.The genes differentially expressed in LB vs LC were mainly concentrated in drug metabolism-cytochrome P450 and glycine,serine and threonine metabolism,PPAR signaling pathway.Salvia miltiorrhiza polysaccharides could be adjusted by the cytochrome P450 metabolic pathways related cytochrome P450 3A4 enzyme (CYP3A4),cytochrome P450 2C23b enzyme (CYP2C23b),glutathione S transferase (GSTA3),kind of glutathione S transferase alpha 2 (GSTAL2) mRNA expression and fatty acid binding protein 1 (FABP1) associated with PPAR signaling pathways and ACSBG2 coa ligase (long chain fatty acids) mRNA expression,thus inhibiting fluorobenzene nicol to produce harmful metabolites,promoting the normal metabolism of liver lipids and alleviating the liver injury caused by flufenicol.

Key words: Salvia miltiorrhiza polysaccharides; florfenicol; broilers; liver injury; transcriptomics

CLC Number:

S853.74

GENG Yumeng, LI Shuying, LU Chunyu, CUI Yuqing, SHI Wanyu, BAO Yongzhan. Study on the Molecular Mechanism of Salvia miltiorrhiza Polysaccharide in Inhibiting Florfenicol-induced Liver Injury in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1849-1858.

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Study on the Molecular Mechanism of Salvia miltiorrhiza Polysaccharide in Inhibiting Florfenicol-induced Liver Injury in Broilers

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