Abstract: In order to observe the effect of different doses of GS-441524 on naturally occurring feline infectious peritonitis(FIP),25 cats diagnosed with FIP in China Agricultural University Veterinary Teaching Hospital were randomly divided into high-dose group (5 mg/(kg·d))(12) and low-dose group (2.5 mg/(kg·d))(13).They were given GS-441524 everyday by subcutaneous injection and recorded daily weight,temperature,pain response of injection and weekly laboratory and imaging examination results.The treatment period was 4 weeks,and some cases were extended according to the actual condition.Finally,20 wet FIP cats and 3 dry FIP cats completed the test,2 cats quit the test.The whole medication was between 4 and 18 weeks.Of the 20 wet FIP cats,2 cats died within 1 week,1 cat's body cavity fluid increased,17 cats' body fluid decreased after 1 week of medication and then disappeared after 2 to 3 weeks of medication.Of the 3 dry FIP cats,2 cats had intestinal membrane lymph nodes that decreased after 1 to 2 weeks of medication,and 1 cat didn't change.The spirit and appetite of all cats improved within 3 to 5 days,weight began to increase within 1 to 2 weeks,5 cats with fever returned to normal after 2 to 3 days,14 anemia cats returned to normal after 1 to 10 weeks,16 cats showed an increase in the number of white blood cells and a decrease in the number of lymphocytes or an increase in the number of neutral granulocytes,and began to improve within 1 to 2 weeks.The ratio of albumin to globulin in all cat serums was less than 0.8,of which 9 cats had an increase in total protein,4 cats had a decrease in albumin,14 cats had an increase in globulin,and began to improve after 1 to 2 weeks.The effective and cure rate of 4 weeks' treatment in high-dose group were 81.8% and 60.0%,respectively.The effective and cure rate of 4 weeks' treatment in the low-dose group were 75.0% and 22.2%,respectively.In addition,statistical results showed significant or extremely significant differences in the indicators of cats in the high-dose group except for white blood cell count and albumin after 4 weeks of medication (P<0.05;P<0.01),while the cat in the low-dose group only had significant weight differences.In addition to hemoglobin,white blood cell count and albumin,the drug had significant differences in weight,red blood cells,red blood cell pressure,lymphocytes,neutral granulocytes,total protein and the ratio of albumin to globulin (P<0.05).Overall,GS-441524 was effective for naturally occurring FIP,and the optimum dosage was 5 mg/(kg·d).

Key words: GS-441524; feline infectious peritonitis (FIP); clinical effects