GPx5在正常及隐睾症双峰驼睾丸及附睾中的作用分析

doi:10.16431/j.cnki.1671-7236.2025.03.020

Abstract

Abstract: 【Objective】 This study was aimed to analyze the distribution and expression of glutathione peroxidase-5 (GPx5) in testis and epididymis of normal and cryptorchidism in Bactrian camels,so as to explore the regulatory effect on reproduction of GPx5 in sperm antioxidant function during cryptorchidism in Bactrian camels.【Method】 The testis and epididymis from adult (5-year-old) Bactrian camels were collected,including 10 pairs of normal group and 6 pairs of cryptorchid group,the histological structural characteristics were observed using HE staining,collagen staining and reticular staining.The expression and distribution of GPx5 were studied by immunohistochemistry staining,Western blotting,immunofluorescence staining,and Image Pro Plus 13.0 image analysis.【Result】 Compared with normal group,Bactrian camels in cryptorchid group showed underdeveloped seminiferous tubules with extremely significantly reduced lumen diameter (P＜0.01),fewer interstitial cells,shrunken lumen with epithelial vacuolation in epididymise,but more abundant reticular and collagen fibers of interstitium in testis and epididymis.The immunohistochemistry results revealed that there was no obvious expression of GPx5 in testis of Bactrian camels in normal group,while there was evident expression in Sertoli cells of Bactrian camels in cryptorchid group.Western blotting results showed that compared with normal group,the expression of GPx5 in testis and corpus epididymidis of Bactrian camels in cryptorchid group was extremely significantly increased (P＜0.01),the expression of GPx5 in caput epididymidis was extremely significantly decreased (P＜0.01),but there was no significant difference in cauda epididymidis (P＞0.05).The results of immunofluorescence localization indicated that compared with normal group,the expression of GPx5 in testis and cauda epididymidis of Bactrian camels in cryptorchid group was extremely significantly increased (P＜0.01),the expression of GPx5 in caput epididymidis was extremely significantly decreased (P＜0.01),but there was no significant difference in corpus epididymidis (P＞0.05).【Conclusion】 The testis and epididymis of cryptorchidism in Bactrian camels showed a tendency towards fibrosis.The expression of GPx5 protein was significantly increased in the testis of cryptorchidism in Bactrian camels,with enhanced expression in Sertoli cells.The oxidative stress in seminiferous tubules affected normal spermatogenesis.The weak expression of GPx5 in the principal cells of caput epididymidis suggested an abnormal antioxidant activity in the epididymal microenvironment,leading to obvious sperm damage.

Key words: glutathione peroxidase-5 (GPx5); Bactrian camels; cryptorchidism; testis; epididymis

CLC Number:

S824

LU Yanan, YUAN Ligang, ZENG Jianlin, QI Yumei, MA Long, LYU Jinhan. Analysis of the Effect of GPx5 on Testis and Epididymis of Normal and Cryptorchidism in Bactrian Camels[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1180-1190.

References

[1] HASI G,WU L,SODNOMPIL T,et al.Differential expression and localisation of heat shock protein 70(HSP70) and glutathione peroxidase 5(GPx5) in the testis and epididymis of Sonid Bactrian camels[J].Reproduction Fertility & Development,2023,35(10):552-562.
[2] PEI J,PAN X,WEI G,et al.Research progress of glutathione peroxidase family (GPx) in redoxidation [J].Frontiers in Pharmacology,2023,14:1147414.
[3] LI L,JIN T,CHEN S,et al.Exploring novel function of GPx5 antioxidant activity:Assisting epididymal cells secrete functional extracellular vesicles [J]. Journal of Cellular Physiology,2024,239(6):e31273.
[4] O’FLAHERTY C,SCARLATA E.Oxidative stress and reproductive function:The protection of mammalian spermatozoa against oxidative stress [J]. Reproduction,2022,164(6):F67-F78.
[5] YANG C,GUO X,DONG F,et al.miR-542-3p reduces antioxidant capacity in goat caput epididymal epithelial cells by targeting glutathione peroxidase 5(GPx5)[J]. Theriogenology,2022,186:168-174.
[6] LUAN Z,FAN X,SONG H,et al.Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro [J].In Vitro Cellular & Developmental Biology-Animal,2019,55(9):677-685.
[7] YANG D,YUAN L,MA X,et al.Histological study of Bactrian camel cryptorchidism and expression of immunoglobulin λ light chain in the testicular and epididymis of cryptorchid Bactrian camel [J].Reproduction in Domestic Animals,2024,59(1):e14512.
[8] 徐志浩.雄性双峰驼生殖系统组织学特征研究[D].兰州：兰州大学，2011. XU Z H.Histological studies on reproductive system in male Bactrian camel (Camelus bactrianus) [D].Lanzhou：Lanzhou University,2011.(in Chinese)
[9] 袁莉刚,张勇,李聪，等.高原地区藏绵羊与小尾寒羊睾丸细胞外基质组织分布特征比较[J].解剖学报，2017,48(2):179-186. YUAN L G,ZHANG Y,LI C,et al.Comparison of distribution characteristics of extracellular matrix components in the testis of the Tibetan sheep and the Small-tail Han sheep from plateau [J].Journal of Anatomy,2017,48(2):179-186.(in Chinese)
[10] CHU C,YU L,HENRY-BERGER J.Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice [J].Asian Journal of Andrology,2020,22(6):590-601.
[11] LI J,BARRANCO I,TVARIJONAVICIUTE A,et al.Seminal plasma antioxidants are directly involved in boar sperm cryotolerance[J].Theriogenology,2018,107:27-35.
[12] 宋娟娟,袁莉刚,王乾梅，等.FGF22及其受体在庆阳黑山羊正常睾丸与隐睾中的表达比较[J].中国畜牧兽医,2021,48(4):1351-1360. SONG J J,YUAN L G,WANG Q M,et al.Comparison of the expression of FGF22 and its receptor in normal testicles and cryptorchidism of Qingyang Black goat[J]. China Animal Husbandry & Veterinary Medicine,2021,48(4):1351-1360.(in Chinese)
[13] RASHAD D E M,IBRAHIM S,EL-SOKARY M M M,et al.Abundance of selected genes implicated in testicular functions in Camelus dromedarius with high and low epididymal semen quality [J].Biology of Reproduction,2024,110(3):501-508.
[14] PARK H J,SONG H,WOO J S,et al.Expression patterns of male germ cell markers in cryptorchid pig testes [J].Acta Histochemica,2019,121(7):784-790.
[15] 常德辉,孔飞燕,罗国雄，等.左卡尼汀对高原环境下大鼠睾丸抗氧化损伤的研究[J].西南国防医药，2021,31(1):8-11. CHANG D H,KONG F Y,LUO G X,et al.Effect of LC on anti-oxidative damage of rat testis in high-altitude environment [J].Medical Journal of National Defending Forces in Southwest China,2021,31(1):8-11.(in Chinese)
[16] DUTTA S,SENGUPTA P,SLAMA P,et al.Oxidative stress,testicular inflammatory pathways,and male reproduction [J].International Journal of Molecular Sciences,2021,22(18):10043.
[17] SRINIVASAN R,HAN H S,SUBRAMANIAN P,et al.Lipid ros- and iron-dependent ferroptotic cell death in unicellular algae Chlamydomonas reinhardtii [J].Cells,2023,12(4):553.
[18] 邢引弟,袁莉刚,陈少宇，等.GPx5在牦牛隐睾附睾组织的分布特征及定位分析[J].云南农业大学学报(自然科学)，2022,37(4):587-596. XING Y D,YUAN L G,CHEN S Y,et al.Distribution characteristics and localization of GPx5 in adult yak cryptorchidic epididymis [J].Journal of Yunnan Agricultural University(Natural Science),2022,37(4):587-596.(in Chinese)
[19] QUAN K,CAO Z,XU Q,et al.A web-based calculator for predicting the prognosis of patients with sarcoma on the basis of antioxidant gene signatures [J].Aging，2022,14(3):1407-1428.
[20] 石一凡,李晓彤,锁云鹏，等.维甲酸对热应激小鼠睾丸损伤的保护作用[J].南京农业大学学报，2024,47(1):183-192. SHI Y F,LI X T,SUO Y P,et al.Protective effect of retinoic acid on testicular injury in heat stressed mice [J].Journal of Nanjing Agricultural University ,2024,47(1):183-192.(in Chinese)
[21] ROMEO C,SANTORO G.Free radicals in adolescent varicocele testis [J].Oxidative Medicine & Cellular Longevity,2014,2014:912878.
[22] 朱江江,袁梦,李鹏程，等.牦牛GPX5基因CDS区的克隆和表达研究[J].中国畜牧杂志，2019,55(10):78-82. ZHU J JG,YUAN M,LI P C,et al.Study on CDS cloning and expression of GPX5 gene in yak [J].Chinese Journal of Animal Science,2019,55(10):78-82.(in Chinese)
[23] MA X,ZHANG B,MIAO R,et al.Transcriptomic and physiological responses to oxidative stress in a Chlamydomonas reinhardtii glutathione peroxidase mutant [J].Genes,2020,11(4):463.
[24] SQUILLACIOTI C,MIRABELLA N,LIGUORI G,et al.Aquaporins are differentially regulated in canine cryptorchid efferent ductules and epididymis [J].Animals (Basel),2021,11(6)：1539.
[25] GAN M,JING Y,XIE Z,et al.Potential function of testicular microRNAs in heat-stress-induced spermatogenesis disorders [J].International Journal of Molecular Sciences,2023,24(10):8809.
[26] 栾兆进,赵勇超,宋慧子，等.绵羊附睾GPX5的抗氧化功能研究[J].西北农林科技大学学报(自然科学版),2022,50(8):8-14. LUAN Z J,ZHAO Y C,SONG H Z,et al.Antioxidant function of GPX5 gene in epididymis of sheep [J].Journal of Northwest A&F University(Natural Science Edition),2022,50(8):8-14.(in Chinese)
[27] 柳苗苗,郭亚军,付德海，等.雄性牦牛和藏绵羊生殖器官的组织学观察[J].实验室科学,2022,25(4):8-11. LIU M M,GUO Y J,FU D H,et al.Histological observation on reproductive organs of male yak and tibetan sheep [J].Laboratory Science,2022,25(4):8-11.(in Chinese)
[28] 刘慧娟,庄苏,张佳琦，等.日粮添加不同水平芦丁对热应激小鼠睾丸组织的影响[J].畜牧兽医学报,2022,53(8):2586-2597. LIU H J,ZHUANG S,ZHANG J Q,et al.Effects of dietary supplementation of different levels of rutin on testicular tissue in mice under heat stress [J].Acta Veterinaria et Zootechnica Sinica,2022,53(8):2586-2597.(in Chinese)

Analysis of the Effect of GPx5 on Testis and Epididymis of Normal and Cryptorchidism in Bactrian Camels

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Siriguleng, YU Wen, JIANG Xiaowei, LI Ziyi, JIN Junjian, BAI Haoyu. The Expression of miR-144-5p in Plasma Exosomes of Different Body Types of Bactrian Camels and the Verification of Target Gene [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1022-1032.
[2]	LI Zhiwei, XIE Xinjie, SHEN Yusong, WANG Hui, XU Ming, ZANG Changjiang, YANG Kailun, LI Fengming. Effects of Different Concentrate Supplementation Levels on Fecal Fermentative Parameters and Microflora Structure of Junggar Bactrian Camels [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1033-1044.
[3]	WANG Zechao, HE Wenying, CHANG Qing, PENG Tao, LI Yanning, BI Fengchen, WANG Junyan, WANG Yin, WANG Lijuan. Effect of Lycium barbarum Polysaccharide on Reproductive Injury Induced by Triptolide in Male Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3719-3729.
[4]	WU Kaihui, ZHOU Chengli, ZHAO Lingjun, XU Shiyuan, LIU Songqi, DONG Zhihao, YUAN Kaimin, WANG Dong. Research Progress on the Mechanism and Regulatory Pathway of Testicular Injury Induced by Busulfan in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2811-2819.
[5]	DU Zhaohui, GE Zihao, YOU Junyi, LIU Xiaowei, LIANG Guodong, SUN Shiduo, HU Jianhong, LI Xiao. Effects of Clostridium butyricum and Sodium Butyricum on Reproductive System Injury Induced by High Fat Diet in Male Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1603-1616.
[6]	WANG Guang, ZOU Jiahao, ZHANG Yongtao, LI Dexian, LI Xueqing, YU Mengqi, CHEN Lu, YUAN Yuxin, LI Guang. Difference Analysis of Metabolites in Testis of Guanzhong Dairy Goats at Different Developmental Stages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3453-3464.
[7]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[8]	HU Wen, HUO Konglin, SONG Xingxing, ZHANG Xin, ZHANG Duoni, LUO Rongrong, XU Wenhao, LI Xun. Effects of Gonadotropin Inhibitory Hormone on Gonadal Reproductive Function and Glucose Metabolism in SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2239-2249.
[9]	LIU Zaixia, DUAN Shi, SUN Yanyong, LYU Qi, FU Shaoyin, HE Xiaolong, ZHANG Wenguang, LIU Yongbin. Study on Differential Gene and Protein Interaction Network of Testis in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 1-11.
[10]	XU Jiaoxia, ZHANG Jiaxin. Research Progress on Antioxidation Protection of Sperm in Epididymis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 91-97.
[11]	SONG Juanjuan, YUAN Ligang, WANG Qianmei, CHEN Shaoyu, GUO Yan. Comparison of the Expression of FGF22 and Its Receptor in Normal Testicles and Cryptorchidism of Qingyang Black Goat [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1351-1360.
[12]	LIU Rui, LI Jiangling, CHEN Xiaohui, ZHAO Sujun, WANG Qiushi, HE Zhiping, WANG Yuping. Identification of circRNA Related to Male Reproduction in Pig Testis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3660-3669.
[13]	REN Jianming, WEI Yanming, YUAN Ligang, WANG Qianmei, LI Chengye. Comparison of the Distribution of PGP9.5 and NPY in Cryptorchidism and Testicular Tumors in Dogs [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2741-2750.
[14]	CHEN Yun, WANG Kai, ZHOU Lele, HUANG Jianhao, YE Chao, WEI Hengxi, LI Li, ZHANG Shouquan. Expression of OPN in Germ Cells of Landrace Boar and Its Correlation with Reproductive Performance [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2536-2543.
[15]	HAN Yue, LIU Hang, CHEN Yang, JIANG Huaizhi. Expression of RBP4 Gene in Epididymis Before and After Sexual Maturation in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 807-813.