枸杞多糖治疗雷公藤甲素诱导雄性小鼠生殖损伤的作用研究

doi:10.16431/j.cnki.1671-7236.2023.09.028

Abstract

Abstract: 【Objective】 This study was aimed to investigate the recovery and therapeutic effect of Lycium barbarum polysaccharide (LBP) on reproductive injury induced by triptolide in male mice,so as to provide clinical basis for the treatment of reproductive injury caused by triptolide and antitoxic compatible drugs for the wide application of triptolide.【Method】 Forty-five SPF grade 4-week-old male ICR mice were randomly divided into 5 groups by digital method:Control group (CON),triptolide injury group (TP),Lycium barbarum polysaccharide low (LBP-L),medium (LBP-M) and high(LBP-H) dose treatment groups.Except for mice in control group were intraperitoneally injected physiological saline,mice in the other groups were intraperitoneally injected with 120 g/kg triptolide,once a day.Two hours later,the CON and TP groups were intragaically irrigated with distilled water,the LBP-L,LBP-M and LBP-H groups was intragaically irrigated with 20,40 and 60 mg/kg Lycium barbarum polysaccharide solution.Mice were killed after consecutive 14 days.Semen quality was measured by semen analyzer,HE staining sections were used to observe the pathological changes of testis in mice.The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),and the contents of malondialdehyde (MDA) and serum testosterone (T) in testis of mice were detected by the kit.The expression of B cell lymphoma-2 (Bcl-2),Bcl-2 associated X protein (Bax) and Cl-Caspase3 protein in testis of mice were detected by Western blotting.【Result】 Compared with CON group,the growth rate of body weight,organ indexes,sperm number,sperm motility and the activities of SOD and GSH-Px in TP group were extremely significantly decreased (P＜0.01).The sperm malformation rate and MDA content were extremely significantly increased (P＜0.01).The results of testicular histopathology showed atrophy of spermatogenic cells,reduction of spermatogenic cells,disorder of cell hierarchy and serious vacuolation structure.Compared with TP group,other indexes except for serum testosterone were improved in different doses of Lycium barbarum polysaccharide groups (P＜0.05 or P＜0.01),and the high dose group had the best improvement effect.Western blotting results showed that compared with CON group,the expression of apoptosis proteins in testis of mice in LBP-H group were significantly or extremely significantly increased (P＜0.05 or P＜0.01).Compared with TP group,the expression of Bax and Cl-Caspase3 proteins and Bax/Bcl-2 ratio in testis of mice in LBP-H group were significantly or extremely significantly decreased (P＜0.05 or P＜0.01),the expression of Bcl-2 protein was significantly extremely increased (P＜0.01).【Conclusion】 Lycium barbarum polysaccharide had a therapeutic effect on the reproductive damage caused by triptolide,and its therapeutic effect was mainly reflected in the increase of antioxidant stress level of organisms,improving sperm quality,alleviating vacuolar cells and improving cell disorders in testis of mice,and inhibiting the apoptosis of testicular cells by regulating the expression of related apoptotic proteins.

Key words: Lycium barbarum polysaccharide; triptolide; reproductive injury; testis; antitoxin compatibility

CLC Number:

S859.8

WANG Zechao, HE Wenying, CHANG Qing, PENG Tao, LI Yanning, BI Fengchen, WANG Junyan, WANG Yin, WANG Lijuan. Effect of Lycium barbarum Polysaccharide on Reproductive Injury Induced by Triptolide in Male Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3719-3729.

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Effect of Lycium barbarum Polysaccharide on Reproductive Injury Induced by Triptolide in Male Mice

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