Abstract:

The study was conducted to investigate the effects of testosterone propionate supplementation on tissues residue of broilers,and its residual effect on serum biochemical parameters,testicular growth and development of broiler chickens,to provide a basis for the protection of animal products safety. A total of 160 male 60-day-old broilers with (2.2±0.1)kg body weight were selected and assigned to control group and testosterone propionate supplementation group. The chicken in control group were fed basal diets while that in testosterone propionate supplementation group were fed diets supplemented with 20 mg/kg testosterone propionate for 7 d. The broilers was slaughtered at the day of 2,6,11 and 17 d after the testosterone propionate supplemented diets were ending. The crest,liver,testis and musle were collected to measure the testosterone propionate residues and testosterone concentration by lquid chromatograph mass spectrometer mass spectrometer (HPLC-MS/MS). The serum samples were collected from the veins to measure the serum biochemical profiles by automatic biochemical analyzer. Testis samples were collected to measure the testis cell morphology and mastocyte accounts by HE and toluidine blue staining. The results showed that:①The testosterone propionate concentration in serum was the highest,followed by the crest. However,there was no testosterone propionate residues in breast muscle,leg muscle,liver and testis. The testosterone propionate concentration in crest was not affected by the withdrawal time (P>0.05). The content of testosterone propionate at 17 d of the withdrawal time was extremely significantly lower than that of 2,6 and 11 d (P<0.01),and there was a regressive between the residues of testosterone propionate in the blood and crest and withdrawal time.②Compared with the control group,the concentration of propionate in testis at the withdrawal time of 2,6,11 and 17 d was reduced 71.45%,63.46%,62.86% and 73.61% (P<0.01),respectively.③The relative weight of testis was significantly lower at the withdrawal time of 2 d (P<0.05). But there was no significant difference in liver relative weights among the two groups in all the withdrawal time (P>0.05).④Most of serum biochemical parameters (TP,ALB,A/G,CHO,TG,LDH,ALT and AST) were not significantly affected by testosterone propionate supplementation (P>0.05).⑤Testis lose the profiles, outed of the order of spermatogenic cells in tubes by testosterone propionate supplementation. Degranulation mass cell accounts,mast cells were higher than control group. In conclusion,the testosterone propionate could result in atrophy of the rooster testis,which could be detected by blood and crest samples,and the results could provide a basis for the study of livestock products safety.

Key words: testosterone propionate; testosterone; testis; residue