China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (2): 454-461.doi: 10.16431/j.cnki.1671-7236.2022.02.006

• Physiological and Biochemical • Previous Articles     Next Articles

Changes of Sex Hormone Levels in the Process of PCOS Induced by DHEA in Mice

WENG Yu1,2, WANG Qin1,2, ZHAO Yufen1,2, YU Kai1,2, HAO HAO Shaoyu1,2, YU Boyang3, DU Chenguang1,2, SUBUDENG Gerile1,2, LI Haijun1,2   

  1. 1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Key Laboratory of Basic Veterinary Medicine, Hohhot 010018, China;
    3. Basic Medical College, Inner Mongolia Medical University, Hohhot 010110, China
  • Received:2021-09-06 Online:2022-02-05 Published:2022-01-27

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Abstract: 【Objective】 In view of the controversy over the pathogenesis and treatment of polycystic ovary syndrome (PCOS), and the limited use of human PCOS materials for research, in the present study, PCOS mice model was constructed for further exploring the changes in sex hormone levels in this process.【Method】 Subcutaneous injection of dehydroepiandrosterone (DHEA) was chosen to produce KM female mice with PCOS-like clinical symptoms, mouse estrous cycle was identified by crystal violet staining.The ovarian development status was determined with HE staining.The changes of serum testosterone and estradiol concentrations during the modeling process were evaluated using ELISA method.【Result】 The vaginal epithelial cells of mice were stained with crystal violet, which could accurately distinguish the different stages of estrous cycle in mice.After 20 days of continuous induction with 6 mg/100 g DHEA, the estrous cycle of KM mice was arrested, and the change of body weight was significantly related to the addition of sesame oil solvent (P < 0.05), but there was no relation to DHEA treatment.After continuous treatment with DHEA, the serum testosterone level increased significantly (P < 0.05), and the serum testosterone level increased significantly from the 5th day of PCOS model establishment and maintained until the end (P < 0.05).The serum estradiol level was significantly higher than that of control group on the 10th and 15th day (P < 0.05), and lower than that of control group on the 20th day.【Conclusion】 The PCOS KM mouse model was successfully established by improving the DHEA dissolution method.During the modeling process, the concentration of androgen continued to be at a high level, while the concentration of estradiol increased at the early stage and decreased at the end of modeling, however, the possible pathological role on estradiol elevated instantly during the modeling process needed to be further verified.

Key words: polycystic ovarian syndrome (PCOS); dehydroepiandrosterone (DHEA); estrous cycle; testosterone; estradiol

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