鸡lncRNA-MSTRG.15568.9及其预测靶基因的表达

doi:10.16431/j.cnki.1671-7236.2019.07.013

Abstract

Abstract:

The objective of this study was to study the spatio-temporal expression of a long non-coding RNA (lncRNA) and its predicted target gene,which are highly expressed in chicken testis,and its regulation in asthenospermia Beijing-You chickens.A high-expression lncRNA (MSTRG.15568.9) was screened by the transcriptome of normal and the asthenospermia Beijing-You chickens.Its potential target gene (sperm-associated antigen 4,SPAG4) was revealed by cis prediction,and RT-qPCR was used to further expression analysis.3 normal cocks with similar body weight were randomly selected at 0,5,20,30,45,and 60 weeks of age to detect the difference in expression between MSTRG.15568.9 and SPAG4 at different ages;At 30 weeks of age,3 normal cocks were selected,and 8 tissues including testis,liver and spleen etc. collected to detect the expression of MSTRG.15568.9 and SPAG4.At 45 weeks of age,3 normal and 3 asthenospermic cocks were selected,and the difference in expression levels between MSTRG.15568.9 and SPAG4 were compared.The results showed that MSTRG.15568.9 and SPAG4 had significant spatio-temporal expression differences,and the expression trends of them were basically the same.The expression trends of MSTRG.15568.9 and SPAG4 were similar in testis tissues of different ages.The expression level of MSTRG.15568.9 at 20 weeks was significantly higher than that at 0,5,30,45 and 60 weeks (P<0.05).The expression levels at 0 and 5 weeks of age were significantly lower than those at 20,30,45 and 60 weeks (P<0.05);SPAG4 was highest at 45 weeks of age,followed by 20 weeks (P<0.05).The expression levels of MSTRG.15568.9 and SPAG4 in testis and liver were significantly higher than that in spleen and kidney (P<0.05).The expression levels of MSTRG.15568.9 and SPAG4 in testis of cocks with normal sperm motility were significantly higher than those in asthenospermia cocks (P<0.05).In conclusion,MSTRG.15568.9 and SPAG4 had obvious tissue expression specificity,and the expression of SPAG4 might be related to MSTRG.15568.9,and both could be involved in spermatogenesis and sperm motility regulation.The specific mechanisms need to be further explored.This study could provide references for the identification of functional genes related to the regulation mechanism of chicken asthenospermia.

Key words: chicken; lncRNA; testis; sperm motility; RT-qPCR

CLC Number:

S831.2

HUANG Ziyan, SUN Yanyan, LI Yunlei, XU Hong, LIU Yifan, SHI Lei, YE Jianhua, MA Hui, NI Aixin, WANG Panlin, Xie Jinfang, CHEN Jilan. Expression of lncRNA-MSTRG.15568.9 and Its Predicted Target Gene in Chicken[J]. , 2019, 46(7): 1986-1993.

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Expression of lncRNA-MSTRG.15568.9 and Its Predicted Target Gene in Chicken

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