无量山乌骨鸡肝脏组织发育特征及脂蛋白脂酶基因表达研究

doi:10.16431/j.cnki.1671-7236.2024.05.020

Abstract

Abstract: 【Objective】 The aim of this study was to study the changes of lipid deposition and lipase activity during the development of liver of Wuliangshan Black-boned chicken and its correlation with lipoprotein lipase(LPL) gene expression.【Method】 In this study,four stages of roosters and hens at 1(D1),42(D42),84(D84) and 126 days of age(D126) were used as the research objects.The body weight and liver weight were measured and the liver index was calculated.HE staining was used to identify the morphological changes of liver.The content of triglyceride (TG) in liver was detected by TG detection kit (single reagent GPO-PAP method).Colorimetric method was used to detect LPL and hepaticlipase (HL) activities.Real-time quantitative PCR was used to detect LPL gene mRNA expression.Pearson correlation analysis was used to determine the correlation between LPL gene expression and liver indexes.【Result】 There were significant differences in liver weight between roosters and hens at different growth stages (P＜0.05).The liver indexes of D42 and D84 were significantly different from those of D1 and D126(P＜0.05).TG content in the liver tissues of both roosters and hens peaked at D84.The activities of LPL and HL in hens reached the peak at D126 and were significantly higher than those at other stages (P＜0.05),and the activities of LPL and HL in roosters reached the peak at D42.The total esterase activity of roosters and hens reached the peak at D42 and D126 respectively and were significantly higher than that at other stages (P＜0.05).The peak of LPL mRNA expression in liver of roosters and hens were at D42 and D1 respectively,and the expression of LPL gene mRNA in hens was significantly correlated with HL activity at three developmental stages of D1,D42 and D126 (P<0.05).【Conclusion】 The liver weight,liver index,TG content,lipase content and LPL gene mRNA expression of Wuliangshan Black-boned chicken varied greatly at different developmental stages.The lipase activity of adult chickens were mainly affected by gender,and the expression of LPL gene mRNA in hens was significantly correlated with HL activity.This results provided a scientific basis for further study on the regulation mechanism of liver fat metabolism in Wuliangshan Black-boned chicken.

Key words: Wuliangshan Black-boned chicken; liver; triglyceride; lipoprotein lipase; hepaticlipase

CLC Number:

S831.2

SHI Yanyan, CHEN Jianzhong, WU Peifu, WU Yun, CHEN Fenfen. Development Characteristics of Liver Tissue and Expression of Lipoprotein Lipase Gene in Wuliangshan Black-boned Chicken[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1988-1997.

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Development Characteristics of Liver Tissue and Expression of Lipoprotein Lipase Gene in Wuliangshan Black-boned Chicken

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