地菍总黄酮对高脂饮食诱导非酒精性脂肪肝大鼠脂代谢的影响

doi:10.16431/j.cnki.1671-7236.2023.08.042

Abstract

Abstract: 【Objective】 This study was conducted to explore the effect of total flavonoids from Melastoma dodecandrum Lour.on lipid metabolism in rats with high fat diet induced nonalcoholic fatty liver disease (NAFLD).【Method】 Sixty SPF male rats were randomly divided into blank group,model group,orlistat group,high,medium and low dose of total flavonoids from Melastoma dodecandrum Lour.groups,with 10 rats in each group.The rats in blank group were fed a basal diet,and the other groups were fed a high fat diet.Orlistat group was given 60 mg/kg orlistat,while high,medium and low dose groups were given 750,500 and 330 mg/kg total flavonoids from Melastoma dodecandrum Lour.,respectively.The corresponding drugs were given by intragastric administration at the volume of 0.1 mL/10 g every day for 42 days.The blank and model group were given equal volume of distilled water.The general status of rats in each group was recorded,and fasting blood glucose (FBG),fasting insulin (FINS),the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the levels of adiponectin (ADP) and leptin (LEP) were detected after administration.Insulin sensitivity index (ISI) and insulin resistance index (HOMA-IR) were analyzed.The contents of triglyceride (TG) and total cholesterol (TC) in liver were detected.Oil red O staining was used to observe the pathological changes of liver.The mRNA and protein expressions of peroxidase proliferator-activated receptor γ (PPARγ),adiponectin receptor 2 (AdipoR2) and p38 in liver of rats in each group were detected by Real-time quantitative PCR and Western blotting,respectively.【Result】 Compared with blank group, the activities of ALT and AST and the levels of LEP, FINS, FBG and HOMA-IR of serum in model group were extremely significantly or significantly increased (P＜0.01 or P＜0.05), the levels of ADP and ISI were extremely significantly decreased (P＜0.01), and more fat was deposited in the liver. The NAFLD model of rat was successfully established. Compared with model group,the activities of ALT and AST in serum and the levels of LEP,FINS,FBG and HOMA-IR in orlistat and high dose of total flavonoids from Melastoma dodecandrum Lour.groups were significantly or extremely significantly decreased (P<0.05 or P<0.01),ADP and ISI levels were extremely significantly increased (P<0.01).The contents of TC and TG in liver of orlistat and high,medium and low dose of total flavonoids from Melastoma dodecandrum Lour.groups were extremely significantly or significantly decreased (P<0.01 or P<0.05),there was less fat deposition in liver,and the mRNA and protein levels of PPARγ,AdipoR2 and p38 were extremely significantly increased (P<0.01).【Conclusion】 Total flavonoids from Melastoma dodecandrum Lour.could significantly improve NAFLD in rats induced by high fat diet,and its mechanism might be related to its involvement in regulating glucolipid metabolism,improving insulin resistance,and regulating PPARγ/AdipoR2/p38 signaling pathway.

Key words: Melastoma dodecandrum Lour.; total flavonoids; nonalcoholic fatty liver disease; lipid metabolism

CLC Number:

R285.5

TANG Yufei, ZHOU Jingkai, MO Yeyun, ZHU Pan, YANG Qiuli, LI Xiaoxiao, LI Li. Effect of Total Flavonoids from Melastoma dodecandrum Lour.on Lipid Metabolism of Nonalcoholic Fatty Liver Rats induced by High-fat Diet[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3438-3447.

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Effect of Total Flavonoids from Melastoma dodecandrum Lour.on Lipid Metabolism of Nonalcoholic Fatty Liver Rats induced by High-fat Diet

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