3种中药成分对玉米赤霉烯酮致小鼠肝脏损伤的缓解作用

doi:10.16431/j.cnki.1671-7236.2024.01.044

Abstract

Abstract: 【Objective】 This study was aimed to explore the relieving effect of traditional Chinese medicine on liver damage caused by zearalenone (ZEA) in animals.【Method】 63 8-week-old Kunming female mice were randomly divided into 7 groups, with 9 mice in each group.In control group, physiological saline was administered orally once in the morning and once in the afternoon;ZEA group, 20 mg/kg ZEA was gavaged in the morning and physiological saline was gavaged in the afternoon.Quercetin, kaempferol, rutin, and compound ingredients groups were traditional Chinese medicine treatment groups.In the morning, 20 mg/kg ZEA was administered by gavage, and in the afternoon, 50 mg/kg quercetin, 50 mg/kg kaempferol, 100 mg/kg rutin, and 100 mg/kg compound ingredients (V_(quercetin):V_(kaempferol):V_(rutin)=1:2:2) were administered by gavage, respectively.Positive control group, 20 mg/kg ZEA were administered by gavage in the morning and 100 mg/kg vitamin E was administered by gavage in the afternoon.Each animal was gavaged with 0.2 mL each time for 7 consecutive days.The mice were euthanized on the 8th day to detect body weight, liver index, serum liver function, antioxidant enzyme activity, and liver inflammation indicators.【Result】 The weight development results showed that compared with control group, ZEA group mice had lower body weight and higher liver weight (P<0.05).Compared with ZEA group, the weight of rutin and compound groups were increased (P<0.05).Compared with control group, the serum liver function test results showed that the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), acid phosphatase (ACP), lactate dehydrogenase (LDH) in ZEA group were increased (P<0.05).Compared with ZEA group, the activity of various enzymes in traditional Chinese medicine and vitamin E treatment groups were decreased (P<0.05).The results of liver oxidative stress test showed that compared with control group, the activity of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) content, and total antioxidant capacity (T-AOC) in liver of ZEA group were decreased (P<0.05), while the content of malondialdehyde (MDA) and LDH were increased (P<0.05).Compared with ZEA group, the activity of SOD, CAT, GSH content and T-AOC in traditional Chinese medicine and vitamin E treatment groups were increased (P<0.05), while the content of MDA and LDH were decreased (P<0.05).Inflammatory factor detection results showed that compared with control group, the content of tumor necrosis factor-α(TNF-α), interleukin-1 β (IL-1β) and IL-6 in ZEA group were increased and the content of IL-10 was decreased (P<0.05). Compared with ZEA group, TNF-α, IL-1β and IL-6 contents of traditional Chinese medicine ingredient and vitamin E treatment groups were decreased (P<0.05), while the IL-10 content were increased in quercetin and vitamin E treatment groups (P<0.05).【Conclusion】 All three traditional Chinese medicine ingredients quercetin, kaempferol, rutin and vitamin E could alleviate the liver damage caused by ZEA in mice, and the effect of kaempferol was the best.

Key words: zearalenone; liver; quercetin; kaempferol; rutin

CLC Number:

S859.3

ZHU Yundi, YANG Qing, WANG Xinfang, LIU Xiaowang, JIANG Guojun. Alleviative Effects of Three Traditional Chinese Medicine Components on Liver Injury in Zearalenone Poisoned Mice[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 434-442.

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Alleviative Effects of Three Traditional Chinese Medicine Components on Liver Injury in Zearalenone Poisoned Mice

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