荷斯坦奶牛乳腺β-防御素的差异表达

Abstract

Abstract: The aim of the present study was to demonstrate the expression of β-defensins in the mammary glands of Holstein cows. Six kinds of β-defensins were amplified and compared the amino acid sequence. The results showed that all of them had six conserved cysteine residues. The nucleic acid sequence was compared with the NCBI database sequence, the homology result of LAP and BNBD4 were 100%, BNBD5 was 99.6%, EBD was 99.5%, BNBD7 and TAP were 98.5%. The mRNA expression of six kinds of defensins was detected by using Real-time quantitative PCR. The results showed that the relative expression of them were significant difference each other, but only the expression of BNBD4 and BNBD5 was no significant difference. The relative expression quantity of LAP was the highest, BNBD7, EBD, BNBD5, BNBD4 were middle, TAP was the lowest.

Key words: β-defensins; differential expression; Holstein cows; mastitis

CLC Number:

Q786

CHENG Lan-ling, CAO Gui-fang, WEN Shi-yong, ZHAO Peng-wei, GUAN Hong-min, JIAN Rui-zhen. Differential Expression of β-defensins from Mammary Gland in Holstein Cow[J]. , 2012, 39(6): 65-68.

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Differential Expression of β-defensins from Mammary Gland in Holstein Cow

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