紫茎泽兰处理鸡柔嫩艾美耳球虫后差异基因筛选和分析

doi:10.16431/j.cnki.1671-7236.2018.09.001

Abstract

Abstract:

To obtain the biological information of differentially expressed genes of Eimeria tenella oocysts treated with Eupatorium adenophorum,0.5% Eupatorium adenophorum were adopted to treat oocyst sporulation process of coccidiosis,suppression subtractive hybridization (SSH) was used to screen differential expression genes of chicken coccidian oocysts after treatment, GO and COG function prediction were used to analyze those genes,and Real-time PCR was used to verify the results of SSH.The results showed that a subtractive cDNA library of chicken Eimeria tenella before and after treatment of Eupatorium adenophorum was successfully constructed,86 ESTs sequences and 31 Unigenes were obtained after splicing and clustering,of which 23 Unigenes were with function annotation,8 Unigenes with no functional annotation,and 4 Unigenes had no homology matching.3 differentially expressed genes were randomly selected to further verify the specificity of the cDNA library by Real-time PCR,and the results showed that the expression of surface antigen 13, 3-hydroxyacyl coenzyme A dehydrogenase and cytochrome P450 genes in Eimeria tenella sporulated oocysts in Eupatorium adenophorum treatment group was obviously lower than that in untreated group,indicating that Eupatorium adenophorum had an inhibitory effect on the activity of oocysts.The main regulation effect of Eupatorium adenophorum on genes were obtained in this study,which laid a theoretical foundation for the development of Eupatorium adenophorum environmental pesticides,and could provide references for the screening of coccidiosis attenuated vaccine or gene deletion vaccine targets.

Key words: chicken Eimeria tenella; Euptorium adenophorum; suppression subtractive hybridization; cDNA library; differential expression genes

CLC Number:

S855.9⁺1

YAN Yuntao, LV Ruiqing, SHI Lianqin, YANG Jianfa, DUAN Gang, ZOU Fengcai, XIANG Xun. Screening and Analysis of Differential Expression Genes in the Eupatorium adenophorum Treatment of Chicken Eimeria tenella[J]. , 2018, 45(9): 2347-2357.

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Screening and Analysis of Differential Expression Genes in the Eupatorium adenophorum Treatment of Chicken Eimeria tenella

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