ST细胞酵母双杂交cDNA文库的构建及鉴定

doi:10.16431/j.cnki.1671-7236.2017.03.007

Abstract

Abstract:

To seek out proteins interact with VP2 structural protein of porcine parvovirus (PPV) in ST cell and study the molecular mechanisms of viral infection and pathogenicity, a ST cDNA yeast hybrid library was created. Firtly,the total RNA of ST was extracted using RNeasy Min Kit,and double strands cDNA was synthesized by SMART technique. Then, the ST cDNA library was successfully created by homologous recombination in yeast, the titer was 8.1×10⁸ CFU/mL, the inserts varied from 250 to 1 000 bp,the average of inserted fragments was about 500 bp,and the recombination rate was 96%.These datas indicated that the yeast two-hybrid cDNA library was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PPV.

Key words: porcine parvovirus (PPV); yeast two-hybird; ST cell; cDNA library

CLC Number:

Q782

WANG Xin, SHI Da, DONG Hui, CAO Li-yan, CHEN Jian-fei, SHI Hong-yan, ZHANG Xin, FENG Li. Construction and Characterization of the Yeast Two-hybrid cDNA Library of ST Cells[J]. , 2017, 44(3): 667-672.

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Construction and Characterization of the Yeast Two-hybrid cDNA Library of ST Cells

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