广灵驴成纤维细胞系的建立及其相关生物学特性研究

doi:10.16431/j.cnki.1671-7236.2018.03.012

Abstract

Abstract:

This study was aimed to establish the fibroblast cell line of Guangling donkey,and protect Guangling donkey at the cell level.In the experiment,the fibroblast cell culture was performed on auricular skin of Guangling donkey using the method of tissue-sticking.The fibroblast cell line was established,and its biological characteristics were studied.The results showed that the shape of most fibroblasts in Guangling donkey were spindle,however,some of them were triangle or star.During the process of culturing,the primary fibroblasts began to dissociate from the edge of the tissue at 4 days of adherence.After 14 days of adherence,the cell confluence rate reached about 80%,and the cells could be subcultured for the first time.The measured cell growth curve which was the typical S-shaped curve showed that the cell growth was in good condition.After cryopreservation,the survival rate decreased.The quantity of chromosomes 2n=62 indicated that the fibroblast cell line of Guangling donkey was successfully established.The fibroblast cell line established by this kind of method provided the foundation for the related research of Guangling donkey.

Key words: Guangling donkey; fibroblast cells; biological characteristics

CLC Number:

S813.3

ZHANG Xu, LE Baoyu, CHENG Zhimin, ZHANG Ningfang, ZHANG Wanfeng, GUO Yulong, LIU Zejun, CAI Mingqi, ZHANG Qiqi, GAO Pengfei, GUO Xiaohong, LI Bugao, CAO Guoqing. Establishment and Biological Characteristics Analysis of Fibroblast Cell Line of Guangling Donkey[J]. , 2018, 45(3): 650-655.

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Establishment and Biological Characteristics Analysis of Fibroblast Cell Line of Guangling Donkey

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