蜂胶黄酮对宿主细胞抗病毒能力的影响

doi:10.16431/j.cnki.1671-7236.2016.08.036

Abstract

Abstract:

This experiment was conducted to study the effects of propolis flavone (PF) on antiviral ability of host cell.First,the maximal safe concentration of PF to CEF and PK-15 was determined by MTT method.Then PF was diluted to five concentrations (from 250 to 15.6 μg/mL) with maintenance medium and acceded to foster system of CEF or PK-15 with NDV,IBDV,TGEV and PPV,respectively,and the cell viability was observed by MTT method at 24,48 and 72 h after infection as the index of high antiviral ability of host cell.The results showed that PF could promote the antiviral ability of host cell.And the effects were related to PF concentration,as the concentration increased,the effects were better.The antiviral activity of PF to NDV and TGEV with envelope in host cell existed in infectious earlier stage,while for IBDV and PPV without envelope,the activity appeared in infectious later stage.The results indicated that the antiviral effect period and mechanism of propolis flavone was different for different viruses,propolis flavone could be use for the prevention of NDV and TGEV,and for the treatment of IBDV and PPV.

Key words: propolis flavone; host cell; antiviral

CLC Number:

S859

GUO Zhen-huan, MA Xia, WANG Tian, SHEN Zhi-qiang. Effects of Propolis Flavone on Antiviral Ability of Host Cell[J]. , 2016, 43(8): 2190-2195.

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Effects of Propolis Flavone on Antiviral Ability of Host Cell

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