山羊PPARα基因克隆、分子特性及组织差异表达分析

doi:10.16431/j.cnki.1671-7236.2018.03.001

Abstract

Abstract:

This study was aimed to clone the CDS of goat peroxisome proliferators-activated receptors-alpha (PPARα) gene,analyze the structure and function of its encoding protein,and discuss its expression pattern in different tissues.In this study,the CDS of PPARα gene was amplified and cloned by RT-PCR,its primary,second and tertiary structures were analyzed by online bioinformatics softwares.Phylogenetic tree was constructed and then used to perform the phylogenetic analysis.Six tissues,including heart,lung,liver,kidney,spleen and omentum,were collected from Jianzhou Da’er goat to determine the relative expression pattern of PPARα by Real-time PCR.The results showed that the CDS of PPARα gene was 1 413 bp in length encoding 470 amino acids,which was a kind of stable hydrophilic protein.Bioinformatics analysis suggested that the second structures of PPARα protein were mainly α-helix and random coil,and 9 potential glycosylation sites and 49 phosphorylation sites were also revealed.The signal peptide and transmembrane domain were not existed in PPARα protein,however,there were obviously conserved DNA-binding and ligand-binding domains.Two different structure enrichment domains were connected by a long chain crimp link and generally consisted of helix structure in the tertiary structure.PPARα amino acid phylogenetic trees indicated that goat had a highly homology with sheep and cattle.PPARα gene mRNA were expressed in all the collected tissues and the highest expression was found in kidney and liver,which were significantly higher than those in the other tissues (P < 0.05).There were moderately relative expression in heart and omentum,while there were the lowest relative expression in lung and spleen.The results showed that the differential expression pattern of PPARα gene in goat might associate with the physiologic function and regulation mechanism,such as fat oxidation,lipid metabolism and anti-oxidative stress.This study would provide a theoretical foundation for further research on physiologic function and regulation mechanism of PPARα gene in goats.

Key words: goat; PPARα gene; clone; bioinformatics; differential expression

CLC Number:

RAO Liaoyuan, ZHOU Jingxuan, WANG Linjie, LI Li, ZHANG Hongping, ZHONG Tao. Cloning, Molecular Characteristics and Differential Expression Pattern of PPARα Gene in Goats (Capra hircus)[J]. , 2018, 45(3): 559-570.

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Cloning, Molecular Characteristics and Differential Expression Pattern of PPARα Gene in Goats (Capra hircus)

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