冷应激对猪脂肪、肝脏组织中细胞因子信号转导抑制因子3 mRNA表达的影响

Abstract

Abstract: The samples of the adipose layer in the neck and bosom, adipose of abdominal cavity, liver of pigs were obtained at 2 h after the temperature of -10 ℃±2 ℃, -5 ℃±2 ℃, 0 ℃±2 ℃, 5 ℃±2℃, 21 ℃±2 ℃ (the control) to measure SOCS3 gene expression by the method of the Real-time PCR in order to evaluate the effect of cold stress on expression of suppressor of cytokine signaling 3(SOCS3) in liver and adipose.The results showed that mRNA of the adipose layer in the neck and bosom, adipose of abdominal cavity, liver of pigs were of significant difference in all the groups(P＜0.05). It was concluded that SOCS3 was sensitive to cold stress which might regulate the balance of adipose and the transduction of signalling pathway related with fat metablisom. This study revealed the role of regulation of SOCS3 on the adipose metabolism of pigs during cold stress, and provided the experimental foundation and theoretical support to study the effect of cold stress and mechanism of this signaling pathway on animal.

Key words: cold stress; SOCS3; adipose; liver

CLC Number:

LIU Ying, YANG Zhan-qing, WEN Jing, YANG Bin, WANG Kai, WU Yong-kui, LIU Ju-xiong, HU Zhong-ming. The Expression of SOCS3 mRNA of Swine in Adipose and Liver during Cold Stress[J]. , 2012, 39(12): 32-36.

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The Expression of SOCS3 mRNA of Swine in Adipose and Liver during Cold Stress

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