Abstract: To found an easy way to isolate and culture myocardial cells of piglets, the hearts were digested by collagenase type Ⅳ and trypsin. The digestion was stopped by adding 10% FBS. Cardiocytes were filtered by 100 item of cells sieve and precipitated by centrifugation, and then added the medium to cardiocytes. Cells were purificated to reduce fibroblast content by adhere bottle and then incubated in the CO₂ incubator. The results showed that the cells were round before adhesion; 12 to 24 hours later, the cells of fusiform and polygon began to adhere and stretch out the parapodium; 3 to 4 days later, cell clusters formed; 5 to 7 days later, the cells grew together flakily. Large cardiocytes with well morphology and high livability could be convenient and quickly obtained by digesting of collagenase type Ⅳ and trypsin.

Key words: cardiocyte; cells culture; piglet