PAGE凝胶电泳银染方法的改进

›› 2009, Vol. 36 ›› Issue (7): 99-101.

• 生物技术 • Previous Articles Next Articles

Advanced Technique for Silver Staining Methods on Polyacrylamide Gels Electrophoresis

ZHANG Jin-cheng，LIANG Yong-wen， FAN Li-na，LIAO He-rong

(College of Animal Science and Technology， Shihezi University， Shihezi 832003， China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
Contact: LIAO He-rong

Abstract

Abstract: To study and modify the optimal methods of silver staining of DNA polyacrylamide gels electrophoresis, polymerase chain reaction (PCR)-polyacrylamide gel electrophoresis silver nitrate (AgNO3) staining were used to detect DNA fragments and pBR322 MspⅠDNA molecular weight. 2 silver staining methods were analyzed and evaluated. The sensitivity of all the band of pBR322 MspⅠ Marker developed was coequally 25 ng. However， comparison of time and step，advanced technique was more convenient. The advanced staining cost only about 25 minutes， and was characterized as simple steps，high sensitivity，little pollution，which was recycled and valuable for practical apply．

Key words: microsatellite; polyacrylamide gel electrophoresis; silver staining

CLC Number:

Q789

ZHANG Jin-cheng;LIANG Yong-wen;FAN Li-na;LIAO He-rong. Advanced Technique for Silver Staining Methods on Polyacrylamide Gels Electrophoresis[J]. , 2009, 36(7): 99-101.

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Advanced Technique for Silver Staining Methods on Polyacrylamide Gels Electrophoresis

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