›› 2009, Vol. 36 ›› Issue (4): 76-79.

• 生物技术 • Previous Articles     Next Articles

Establishment and Application of Fluorescence Quantitative TaqMan-MGB PCR Assay for Pseudorabies Virus

LIU Yuan-yuan1, WU Hui1, XIAO Xing-long1,2, JIA Yun3,WU Bin3,YU Yi-gang1   

  1. (1.College of Light Industry & Food, South China University of Technology, Guangzhou 510640,China; 2.Shenzhen Taitai Genomics, Inc., Shenzhen 518057,China;3. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-04-20 Published:2009-04-20
  • Contact: XIAO Xing-long

Abstract: A pair of specific primers and one TaqMan-MGB probe were designed based on gE gene through comparison and analysis, the FQ-PCR method was successfully constructed which could distinguish wild strain and gene-deleted vaccine strain. This method had the characteristic of high specificity to differentiate PRV form other virus. A comparison between FQ-PCR assay and conventional PCR assay were performed with 83 tissue samples, results showed that two methods match well. It indicates that TaqMan-MGB FQ-PCR assay provides a good method to detect PRV.

Key words: pseudorabies virus(PRV); fluorescence quantitative PCR(FQ-PCR); detect

CLC Number: