To protect Procapra praprzewalskii appointed as CR (Critically Endangered), twenty pairs of microsatellite primers from Granti’s gazelle and sheep separately were used to identify the individual of przewalskii gazelle through PCR method, we found that 8 loci were polymorphic. The values of gene heterozygosity is 0.71~0.84, the values of effective number of alleles is 3.40~6.59. At the same time, we took advantage of the 8 loci to identify 35 individuals from 39 faecal samples from Procapra praprzewalskii