利用微卫星标记分析山西3个地方牛群体的遗传多样性和遗传分化

doi:10.16431/j.cnki.1671-7236.2025.01.020

Abstract

Abstract: 【Objective】 This study was aimed to investigate the genetic diversity,population genetic structure and genetic differentiations of Jinnan cattle,Pinglu Mountain cattle and "Taihang Yun cattle",so as to provide data bases for the protection of local cattle in Shanxi province. 【Method】 12 microsatellite (SSR) markers were selected to perform PCR amplification,sequencing and genotyping in the 3 populations(including 230 individuals).At the same time,the allele numbers,genetic heterozygosity,polymorphic information content (PIC) and genetic distance between populations were detected and calculated. 【Result】 The allele numbers of 12 SSR markers were 2 to 15.Besides,the average of effective number of alleles (Ne),observation of heterozygosity (Ho),expected heterozygosity (He) and polymorphism information content (PIC) of 3 populations were from 3.025 to 4.611,0.680 to 0.735,0.646 to 0.750 and 0.601 to 0.716,respectively.Hardy-Weinberg equilibrium (HWE) results showed that except for HAUT27 was deviated from HWE (P＜0.05) in Jinnan population,the other SSR markers were all in HWE (P＞0.05).F statistics analysis showed that the within population inbreeding coefficient (F_IS),population average inbreeding coefficient (F_IT) and population differentiation coefficient (F_ST) were ―0.012, 0.043 and 0.054,respectively.The UPGMA phylogenetic tree indicated that Jinnan cattle and Pinglu Mountain cattle were clustered firstly,then clustered with "Taihang Yun cattle".The principal coordinate analysis (PCoA) results showed that there was closer relationship between Jinnan cattle and Pinglu Mountain cattle,with genetic exchanges existing between the two populations.The STRUCUTRE analysis results found that there were population admixture among 3 populations. 【Conclusion】 The genetic polymorphisms of 3 local cattle populations in Shanxi province were higher,and they all had relatively independent phylogenetic relationships.There was more genetic exchanges between Jinnan cattle and Pinglu Mountain cattle.The results contributed to a deeper understanding of the genetic structure of 3 local cattle breeds in Shanxi province,and provided data basis for the formulation of variety protection plans.

Key words: local cattle populations; microsatellite; genetic diversity; genetic differentiation; genetic structure

CLC Number:

S813.3

LI Chaojie, MENG Meng, LI Bo, JIN Guang, WANG Kun, CHE Leijie, QIAO Xiaochun, ZHANG Yuanqing, NIU Xiaoyan. Analysis of Genetic Diversity and Genetic Differentiations in 3 Local Cattle Populations of Shanxi Province Using Microsatellite Markers[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 226-237.

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Analysis of Genetic Diversity and Genetic Differentiations in 3 Local Cattle Populations of Shanxi Province Using Microsatellite Markers

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