荷斯坦母牛TLR10基因PCR-SSCP分析

›› 2009, Vol. 36 ›› Issue (12): 81-83.

• 遗传繁育 • Previous Articles Next Articles

CR-SSCP Analysis on TLR10 Gene in Holstein Cows

LI Tuo¹, CHU Ming-xing², WANG Jin-yu¹, AN Yong-fu³, DI Ran², FANG Li²

(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009,China; 2.Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193,China; 3.Hebei Animal Science and Veterinary Medicine Institute, Baoding 071000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
Contact: CHU Ming-xing

Abstract

Abstract: The TLR10 (Toll-like receptor 10) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows. Polymorphism of TLR10 gene was detected with fifteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 210 Holstein cows. No polymorphism was detected in the amplified region, and this result indicated that the genetic markers that affect mastitis resistance in Holstein cows may not exist in the detected region of TLR10 gene.

Key words: Holstein cows; TLR10 gene; PCR-SSCP

CLC Number:

LI Tuo;CHU Ming-xing;WANG Jin-yu;AN Yong-fu;DI Ran;FANG Li. CR-SSCP Analysis on TLR10 Gene in Holstein Cows[J]. , 2009, 36(12): 81-83.

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CR-SSCP Analysis on TLR10 Gene in Holstein Cows

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