荷斯坦母牛AHCY基因PCR-SSCP分析

›› 2009, Vol. 36 ›› Issue (11): 93-96.

• 遗传繁育 • Previous Articles Next Articles

PCR-SSCP Analysis on AHCY Gene in Holstein Cows

LI Tuo¹, CHU Ming-xing², WANG Jin-yu¹, AN Yong-fu³, DI Ran², FANG Li²

(1．College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2．Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China； 3．Hebei Animal Science and Veterinary Medicine Institute, Baoding 071000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-11-20 Published:2009-11-20
Contact: CHU Ming-xing

Abstract

Abstract: The AHCY (S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows. Single nucleotide polymorphisms (SNPs) of all exons (exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 210 Holstein cows. No polymorphism was detected in the amplified region in the ten exons tested, this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

Key words: Holstein cows; AHCY gene; PCR-SSCP

CLC Number:

LI Tuo;CHU Ming-xing;WANG Jin-yu;AN Yong-fu;DI Ran;FANG Li. PCR-SSCP Analysis on AHCY Gene in Holstein Cows[J]. , 2009, 36(11): 93-96.

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PCR-SSCP Analysis on AHCY Gene in Holstein Cows

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