›› 2007, Vol. 34 ›› Issue (7): 83-85.

• 疾病防治 • Previous Articles     Next Articles

Detection of Infectious Laryngotracheitis Virus with Polymerase Chain Reaction

CHEN Hongying1,2， LI Xinsheng1，ZHANG Hongying1， FANG Zhongyi3，WANG Dongfang1,2，CUI Baoan1，2
   

  1. 1.College of Animal Husbandry and Veterinary，Henan Agricultural University， Zhengzhou 450002，China； 2.Animal Food Safety Key Laboratory，Henan Province，Zhengzhou 450002，China； 3.Henan Institute of Veterinary Drug Control ，Zhengzhou 450002，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-20 Published:2007-07-20

Abstract: One pair of primers was designed and synthesized according to the chicken infectious laryngotracheitis virus (ILTV) TK gene nucleotide sequence in the Genbank. When PCR was performed with 2 virulent ILTV，1 vaccine ILTV and other pathogenic microorganisms, the amplified fragment of ILTV was about 1183 bp which could be confirmed by sequencing analysis and restriction endonuclease analysis, others were negative．21 pg ILTV DNA could be detected by PCR．All of the tracheal sponge samples from experimentally infected chickens was amplified a specific 1183 bp fragment of TK gene． The results suggested that the method can be used for detection and diagnosis of ILTV in clinic samples．

Key words: ILTV; detection; PCR

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