基因修饰技术及其在动物育种和生物医药领域的应用

doi:10.16431/j.cnki.1671-7236.2024.02.022

Abstract

Abstract: Gene modification is a technology that can be used to achieve site-specific integration of exogenose and knockout of endogenous genes. The early form of gene modification was mainly transgene.With the deepening of scientific research, new gene modification methods were gradually developed, such as knockout, knock in, site-specific mutation, etc.According to the purpose of research or application, gene modification technology can be divided into transgenic and gene knockout.In recent years, with the rapid development of modern molecular technology, gene modification technology has been continuously improved and innovated, and its related methods and technologies have been gradually applied in the fields of improving livestock traits, studying gene function, making animal bioreactors and constructing animal models of human diseases, making the research of gene function and transgenic breeding of livestock and poultry more efficient.Remarkable achievements have been made in the fields of animal genetic breeding and biomedicine, which have made up for the defects of random integration and genetic instability of traditional transgenic technology, and have broad prospects for development.In this paper, the development status and trend of gene modification technology were described from the aspects of animal transgenic and gene knockout technologies, and the application status of gene modification technology in the field of animal breeding and biomedicine was briefly summarized.

Key words: gene modification; transgenic technology; gene knockout technology

CLC Number:

S813.3

YAN Yanxia, LI Zicong, DONG Yazheng, LI Zheng, HUANG Sixiu. Gene Modification Technology and Its Application in Animal Breeding and Biomedicine[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 659-667.

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Gene Modification Technology and Its Application in Animal Breeding and Biomedicine

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