China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 659-667.doi: 10.16431/j.cnki.1671-7236.2024.02.022

• Genetics and Breeding • Previous Articles     Next Articles

Gene Modification Technology and Its Application in Animal Breeding and Biomedicine

YAN Yanxia1,2, LI Zicong1,2, DONG Yazheng1,2, LI Zheng1,2, HUANG Sixiu1,2   

  1. 1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
    2. National Engineering Research Center for Breeding Swine Industry, Guangzhou 510642, China
  • Received:2023-09-25 Online:2024-02-05 Published:2024-01-29
  • Contact: 广东省乡村振兴战略专项"广东省畜禽地方品种保护与开发利用提升工程"
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: Gene modification is a technology that can be used to achieve site-specific integration of exogenose and knockout of endogenous genes. The early form of gene modification was mainly transgene.With the deepening of scientific research, new gene modification methods were gradually developed, such as knockout, knock in, site-specific mutation, etc.According to the purpose of research or application, gene modification technology can be divided into transgenic and gene knockout.In recent years, with the rapid development of modern molecular technology, gene modification technology has been continuously improved and innovated, and its related methods and technologies have been gradually applied in the fields of improving livestock traits, studying gene function, making animal bioreactors and constructing animal models of human diseases, making the research of gene function and transgenic breeding of livestock and poultry more efficient.Remarkable achievements have been made in the fields of animal genetic breeding and biomedicine, which have made up for the defects of random integration and genetic instability of traditional transgenic technology, and have broad prospects for development.In this paper, the development status and trend of gene modification technology were described from the aspects of animal transgenic and gene knockout technologies, and the application status of gene modification technology in the field of animal breeding and biomedicine was briefly summarized.

Key words: gene modification; transgenic technology; gene knockout technology

CLC Number: