晋汾白猪ApoA5基因单倍型与体重和肉质性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.11.018

Abstract

Abstract: This study was aimed to explore the correlation between the haplotypes of apolipoprotein A5 (ApoA5) gene and body weight,meat quality in Jinfen White pigs.180 Jinfen White pigs were selected,PCR was used to detect the single nucleotide polymorphism (SNP) of ApoA5 gene,and the linkage of these SNPs was analyzed by Haploview software.Non-fully linked SNPs were screened to form the haplotypes of ApoA5 gene,and the frequency of different haplotypes of ApoA5 gene were calculated.The correlation between the haplotypes of ApoA5 gene and body weight,meat quality was analyzed by SPSS software to identify the haplotypes of ApoA5 gene with excellent traits.The results showed that ApoA5 gene of Jinfen White pigs contained 21 SNPs and 5 completely linked regions which included 5,2,3,4 and 2 SNPs,respectively.Ten non-completely linked tag SNPs were selected to form the haplotypes of ApoA5 gene,Hap 1 had the highest gene frequency (46.7%).The haplotypes of ApoA5 gene had a significant correlation with the birth weight,weaning weight,6-month-old body weight,intramuscular fat content,shear force and drip loss,but no significant correlation with pH_{24 h} in Jinfen White pigs.Hap 11 of ApoA5 gene in Jinfen White pigs had the highest birth weight,weaning weight,6-month-old body weight and intramuscular fat content,the lowest shear force,and the lower drip loss.Hap 6 had the lowest birth weight.Hap 2 had the lowest weaning and 6-month-old weight.Hap 3 had the lowest intramuscular fat content and the highest shear force.Hap 10 had the highest drip loss.Hap 11 of ApoA5 gene in Jinfen White pigs had excellent traits,which could be used as a molecular marker to use in molecular breeding of Jinfen White pigs and improve the breeding efficiency.

Key words: Jinfen White pigs; ApoA5 gene; SNP; haplotype; body weight; meat quality

CLC Number:

S813.3

CAI Chunbo, XU Kai, LI Meng, YANG Yang, ZHAO Yan, GAO Pengfei, GUO Xiaohong, CAO Guoqing, LI Bugao. Correlation Analysis Between Haplotypes of ApoA5 Gene on Body Weight and Meat Quality in Jinfen White Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3563-3572.

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Correlation Analysis Between Haplotypes of ApoA5 Gene on Body Weight and Meat Quality in Jinfen White Pigs

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