响应面法优化微生物除臭效果的研究

doi:10.16431/j.cnki.1671-7236.2020.08.039

Abstract

Abstract: In order to reduce the odor pollution caused by livestock and poultry manure,four species of microbes with deodorization function such as Bacillus velezensis, Bacillus subtilis,Candida utilis and Lactobacillus casei were selected as 4 factors of experimental design in this study.The corresponding viable counts used in pig manure were 1×10⁴,1×10⁵,and 1×10⁶ CFU/g as the three coding levels of the response surface design.In order to obtain an ideal microbial inoculation proportion for fecal deodorization,4 factors and 3 levels were used to construct 29 combinations of microorganisms in Box-Behnke design for removing indole,NH₃ and H₂S in feces.The results showed that the optimal ratio of Bacillus velezensis,Bacillus subtilis,Candida utilis and Lactobacillus casei obtained by response surface optimization were 0.47%,0.05%,0.01% and 1.00% at the concentration of 1×10⁸ CFU/mL,respectively.After 7 d microbial fermentation,the removal rates of indole,NH₃ and H₂S in pig feces were 73.59%,63.60% and 70.29%,respectively.It could be concluded that an ideal compound microbial preparation for pig fecal innocent treatment had been obtained in this study,which provided the high application value to control the odor pollution caused by livestock and poultry manure.

Key words: manure; indole; ammonia; hydrogen sulfide; microbial fermentation; deodorization

CLC Number:

S811

SHAO Shuan, DANG Xiaowei, LI Huijuan, CHANG Juan, WANG Ping, YIN Qingqiang, GAO Tianzeng. Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2684-2693.

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Study on Optimizing the Effectiveness of Microbial Deodorization by Response Surface Methodology

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