球虫消毒剂的筛选及氨水对艾美耳球虫卵囊抑杀条件的优化

doi:10.16431/j.cnki.1671-7236.2018.12.035

Abstract

Abstract:

To screen some effective disinfectants against coccidian,oocysts of Eimeria tenella had been suspended and maintained in diluted 16 common disinfectants and 1 anti-coccidial drug (250 mg/L toltrazuril) for 2 or 4 h,and then sporulated in 2.5% potassium dichromate solution at 28℃ or inoculated into coccidian-free chickens at 14 days of age.Disinfection efficacy of these disinfectants against both unsporulated and sporulated oocysts were evaluated through oocyst sporulation rates,inhibitory rates of sporulation,OPG values,cecal lesion score and blood stool level in this study.The results of inhibitory efficacy tests of disinfectants and anti-coccidial drugs against both unsporulated and sporulated oocysts showed that 8% ammonia hydroxide could 100% inhibit the unsporulated and sporulated oocysts.250 mg/L toltrazuril,3% formaldehyde,4% phenol,0.5% peroxyacetic acid,5% NaOH and 5% NaOH+10% NaCl solutions showed partial inhibitory activities.Although inhibitory rates of sporulation of these six disinfectants were more than 10%,the inhibitory efficacy of them against sporulated oocysts were not significant.However,the remaining 10 disinfectants didn't have significant inhibitory activities against oocysts.The optimization results of inhibitory conditions of ammonia hydroxide showed that 1% to 8% ammonia hydroxide mixed with unsporulated oocysts for more than 1 h were able to 100% inhibit the activities of oocysts.In addition,the anti-coccidial efficacy of 2% and 4% ammonia hydroxide could not be interfered by animal feces.In conclusion,almost all common disinfectants and anti-coccidial drugs fail to effectively inhibit the activities of coccidian oocysts,while ammonia hydroxide was a highly effective disinfectant.These findings would provide important references for development of novel effective coccidian disinfectants and control of coccidiosis in livestock.

Key words: coccidian oocysts; common disinfectants; ammonia hydroxide; sporulation rates of oocysts; inhibition rates of sporulation

CLC Number:

S852.72

HAN Lifang, YAN Wenchao, LI Yayun, DU Tingting, QIAN Weifeng, ZHANG Min, WEI Zhiguo, WANG Tianqi. Screening of Disinfectants Against Coccidian and Optimization of Inhibitory Conditions of Ammonia Hydroxide Against Oocysts of Eimeria tenella[J]. , 2018, 45(12): 3601-3607.

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Screening of Disinfectants Against Coccidian and Optimization of Inhibitory Conditions of Ammonia Hydroxide Against Oocysts of Eimeria tenella

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