海藻硫酸多糖和中药复方对鸡胚接种H9N2亚型禽流感病毒的作用

doi:10.16431/j.cnki.1671-7236.2018.12.034

Abstract

Abstract:

To explore the effects of sulfated polysaccharide from algae (SPA) and the compound extracts of Chinese medicine (CECM) on H9N2 subtype avian influenza virus (AIV),SPA and CECM were applied to the 10-day-old SPF chicken embryos inoculated with H9N2 subtype AIV in 3 different ways.The chicken embryo allantoic fluid was collected aseptically,the blood coagulation titer was measured,and the prevention,treatment and direct inactivation of the two kinds of liquids to H9N2 subtype AIV were tested.The results showed that the maximum safe concentrations of SPA and CECM were 50 and 200 mg/mL,respectively.Under different dosing methods,different concentrations of SPA and CECM could extremely significantly reduce the blood coagulation of H9N2 subtype AIV in chicken embryo allantoic fluid (P<0.01).The hemocoagulation titer of the direct inactivation group decreased the most,the preventive group and the therapeutic group followed.In the preventive group,the anti-viral effect of CECM high dose group (200 mg/mL) was significantly better than that of SPA dose groups (12.5,25,50 mg/mL) and CECM low and medium dose groups (50,100 mg/mL) (P<0.05);SPA high-dose group (50 mg/mL) and CECM medium-and high-dose groups (100,200 mg/mL) were significantly better than SPA low group (12.5 mg/mL) (P<0.05);The direct inactivation group,SPA and CECM both had good antiviral effects,and the antiviral effects of CECM dose groups were extremely significantly different from those of SPA dose groups (P<0.01).The results showed that SPA and CECM had almost no toxic side effects on 10 days old chicken embryos;SPA and CECM could significantly inhibit the proliferation of H9N2 subtype AIV in chicken embryo allantoic fluid,and the antiviral effect of direct inactivation group was the best.The strength of its anti-H9N2 subtype AIV was closely related to the drug concentration,medication and viral infection time sequence.

Key words: sulfated polysaccharide from algae (SPA); compound extracts of Chinese medicine (CECM); H9N2 subtype avian influenza virus; chicken embryo

CLC Number:

S853.7

CHENG Dengfang, WANG Hongjun, HUANG Qingzhou, ZHANG Li, LIU Juan, XIAN Hong. Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo[J]. , 2018, 45(12): 3594-3600.

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Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo

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